Piezo1 Is as a Novel Trefoil Factor Family 1 Binding Protein that Promotes Gastric Cancer Cell Mobility In Vitro

CORC > 厦门大学 > 医学院－已发表论文

	Piezo1 Is as a Novel Trefoil Factor Family 1 Binding Protein that Promotes Gastric Cancer Cell Mobility In Vitro
	Yang, Xiao-Ning ; Lu, Ya-Pi ; Liu, Jing-Jing ; Huang, Jian-Kun ; Liu, Yun-Peng ; Xiao, Chuan-Xing ; Jazag, Amarsanaa ; Ren, Jian-Lin ; Guleng, Bayasi ; Ren JL(任建林) ; 巴亚斯古楞
刊名	http://dx.doi.org/10.1007/s10620-014-3044-3
	2014
关键词	SIGNALING PATHWAYS BETA-CATENIN GROWTH ACTIVATION EXPRESSION PEPTIDES RECEPTOR GENE INTEGRINS MIGRATION
英文摘要	National Natural Science Foundation of China [81370505, 81370591, 91229201]; Key Projects for Technology Plan of Xiamen [3502Z20100002]; Ministry of Health Foundation for State Key Clinical Department in China; Trefoil factor family 1 (TFF1) is a member of the TFF-domain peptide family involved in epithelial restitution and cell motility. Recently, we screened Piezo1 as a candidate TFF1-binding protein. We aimed to confirm Piezo1 as a novel TFF1 binding protein and to assess the role of this interaction in mediating gastric cancer cell mobility. This interaction was confirmed by co-immunoprecipitation and co-localisation of TFF1 and Piezo1 in GES-1 cells. We used stable RNA interference to knockdown Piezo1 protein expression and restored the expression of TFF1 in the gastric cancer cell lines SGC-7901 and BGC-823. Cell motility was evaluated using invasion assay and migration assay in vitro. The expression levels of the integrin subunits beta 1, beta 5, alpha 1 as well as the expression of beta-catenin and E-cadherin were detected by Western blot. We demonstrate that TFF1, but not TFF2 or TFF3, bind to and co-localize with Piezo1 in the cytoplasm in vitro. TFF1 interacts with the C-terminal portion of the Piezo1 protein. Wound healing and trans-well assays demonstrated that the restored expression of TFF1 promoted cell mobility in gastric cancer cells, and this effect was attenuated by the knockdown of Piezo1. Western blots demonstrated the decreased expression of integrin beta 1 in Piezo1-knockdown cells. Our data demonstrate that Piezo1 is a novel TFF1 binding protein that is important for TFF1-mediated cell migration and suggest that this interaction may be a therapeutic target in the invasion and metastasis of gastric cancer.
语种	英语
出版者	SPRINGER
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93592]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Yang, Xiao-Ning,Lu, Ya-Pi,Liu, Jing-Jing,et al. Piezo1 Is as a Novel Trefoil Factor Family 1 Binding Protein that Promotes Gastric Cancer Cell Mobility In Vitro[J]. http://dx.doi.org/10.1007/s10620-014-3044-3,2014.
APA	Yang, Xiao-Ning.,Lu, Ya-Pi.,Liu, Jing-Jing.,Huang, Jian-Kun.,Liu, Yun-Peng.,...&巴亚斯古楞.(2014).Piezo1 Is as a Novel Trefoil Factor Family 1 Binding Protein that Promotes Gastric Cancer Cell Mobility In Vitro.http://dx.doi.org/10.1007/s10620-014-3044-3.
MLA	Yang, Xiao-Ning,et al."Piezo1 Is as a Novel Trefoil Factor Family 1 Binding Protein that Promotes Gastric Cancer Cell Mobility In Vitro".http://dx.doi.org/10.1007/s10620-014-3044-3 (2014).