A sandwich-type DNA biosensor based on electrochemical co-reduction synthesis of graphene-three dimensional nanostructure gold nanocomposite films | |
Liu, Ai-Lin ; Zhong, Guang-Xian ; Chen, Jin-Yuan ; Weng, Shao-Huang ; Huang, Hong-Nan ; Chen, Wei ; Lin, Li-Qing ; Lei, Yun ; Fu, Fei-Huan ; Sun, Zhou-liang ; Lin, Xin-Hua ; Lin, Jian-Hua ; Yang, Shu-Yu ; Yang SY(杨叔禹) | |
刊名 | http://dx.doi.org/10.1016/j.aca.2012.12.049 |
2013 | |
关键词 | ACUTE PROMYELOCYTIC LEUKEMIA SEQUENCE-SPECIFIC DETECTION BUBBLE DYNAMIC TEMPLATE LITHIUM ION BATTERIES PLATINUM NANOPARTICLES CARBON NANOTUBES FEMTOMOLAR DNA SURVIVIN OXIDE OSTEOSARCOMA |
英文摘要 | National High Technology and Development of China [2012AA022604]; Nationnal Natural Science Foundation of China [20975021, 21175023, 81171668, 21275028]; Fujian Provincial University-Industry Cooperation Sciecne & Technology Major Program [2010Y4003]; Foundation of Fujian Key Laboratory of Hematology [2009J1004]; Scientific Research Major Program of Fujian Medical University [09ZD013]; Natural Science Foundation of Fujian Province of China [2010J06011]; Foundation of Fujian Provincial Department of Education [JA10126, JA11110, JA12130]; A novel electrochemical DNA biosensor based on graphene-three dimensional nanostructure gold nanocomposite modified glassy carbon electrode (G-3D Au/GCE) was fabricated for detection of survivin gene which was correlated with osteosarcoma. The G-3D Au film was prepared with one-step electrochemical coreduction with graphite oxide and HAuCl4 at cathodic potentials. The active surface area of G-3D Au/GCE was 2.629 cm(2), which was about 3.8 times compared to that of a Au-coated GCE under the same experimental conditions, and 8.8 times compared to a planar gold electrode with a similar geometric area. The resultant nanocomposites with high conductivity, electrocatalysis and biocompatibility were characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). A "sandwich-type" detection strategy was employed in this electrochemical DNA biosensor and the response of this DNA biosensor was measured by CV and amperometric current-time curve detection. Under optimum conditions, there was a good linear relationship between the current signal and the logarithmic function of complementary DNA concentration in a range of 50-5000 fM with a detection limit of 3.4 fM. This new biosensor exhibited a fast amperometric response, high sensitivity and selectivity and has been used in a polymerase chain reaction assay of real-life sample with a satisfactory result. (C) 2013 Elsevier B.V. All rights reserved. |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE BV |
内容类型 | 期刊论文 |
源URL | [http://dspace.xmu.edu.cn/handle/2288/93577] |
专题 | 医学院-已发表论文 |
推荐引用方式 GB/T 7714 | Liu, Ai-Lin,Zhong, Guang-Xian,Chen, Jin-Yuan,et al. A sandwich-type DNA biosensor based on electrochemical co-reduction synthesis of graphene-three dimensional nanostructure gold nanocomposite films[J]. http://dx.doi.org/10.1016/j.aca.2012.12.049,2013. |
APA | Liu, Ai-Lin.,Zhong, Guang-Xian.,Chen, Jin-Yuan.,Weng, Shao-Huang.,Huang, Hong-Nan.,...&杨叔禹.(2013).A sandwich-type DNA biosensor based on electrochemical co-reduction synthesis of graphene-three dimensional nanostructure gold nanocomposite films.http://dx.doi.org/10.1016/j.aca.2012.12.049. |
MLA | Liu, Ai-Lin,et al."A sandwich-type DNA biosensor based on electrochemical co-reduction synthesis of graphene-three dimensional nanostructure gold nanocomposite films".http://dx.doi.org/10.1016/j.aca.2012.12.049 (2013). |
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