The PI3K/Akt and ERK1/2 signaling pathways mediate the erythropoietin-modulated calcium influx in kainic acid-induced epilepsy

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	The PI3K/Akt and ERK1/2 signaling pathways mediate the erythropoietin-modulated calcium influx in kainic acid-induced epilepsy
	Zheng, Honghua ; Wang, Xiaojing ; Tang, Zhengang ; Zheng, Weihong ; Li, Zhengli ; Zheng HH(郑红花)
刊名	http://dx.doi.org/10.1097/WNR.0b013e32835ffe03
	2013
关键词	NEURONS IN-VITRO ACTIVATED MICROGLIA ACQUIRED EPILEPSY RAT HIPPOCAMPAL RECEPTOR VIVO EXCITOTOXICITY INJURY
英文摘要	Natural Science Research Foundation of China [81 100842, 81171117]; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry [44]; Natural Science Foundation of Fujian Province of China [2012J01418]; Basic Foundation of Government [ZK1014]; Erythropoietin (EPO) suppresses epileptogenesis and limits the neuronal damage associated with recurrent seizures, but the neurocellular mechanism is unclear. Dysregulation of intracellular calcium homeostasis is a key pathogenic event leading to the progression of epileptic activity, suggesting that EPO may suppress seizures by stabilizing intracellular calcium. In this study, we examined the effects of EPO on voltage-gated Ca2+ influx in cultured rat hippocampal neurons and population spike (PS) amplitude in kainic acid (KA)-induced rats and the mechanisms responsible. KA injection markedly increased EPO and EPO receptor expression and the amplitude of PS in the hippocampal CA3 region, evoked by perforant pathway stimulation. Intracerebroventricular injection of exogenous rat recombinant EPO reversed KA-induced PS amplitude in the hippocampal CA3 region. Similarly, rat recombinant EPO pretreatment attenuates the increased voltage-gated calcium current's (I-Ca) amplitude and density induced by KA in cultured hippocampal neurons. In contrast, transient transfection of rat EPO small interfering RNS (siRNA) further enhanced I-Ca amplitude and density in the presence of KA, whereas a scrambled control siRNA had no effect. Further, EPO activates the PI3K and ERK1/2 pathways in cultured hippocampal neurons, and the PI3K/Akt inhibitor LY294002 and ERK1/2 inhibitor U0126 both blocked, at least in part, the suppressive effect of exogenous EPO on KA-induced calcium currents. This study indicates that both endogenous and exogenous EPO decrease KA-sensitive calcium influx and concomitant hyperexcitability in hippocampal neurons. The results also demonstrate that the PI3K/Akt and ERK1/2 signaling pathways mediate the EPO-modulated calcium influx in KA-induced epilepsy. NeuroReport 24:335-341 (C) 2013 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
语种	英语
出版者	LIPPINCOTT WILLIAMS & WILKINS
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93554]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Zheng, Honghua,Wang, Xiaojing,Tang, Zhengang,et al. The PI3K/Akt and ERK1/2 signaling pathways mediate the erythropoietin-modulated calcium influx in kainic acid-induced epilepsy[J]. http://dx.doi.org/10.1097/WNR.0b013e32835ffe03,2013.
APA	Zheng, Honghua,Wang, Xiaojing,Tang, Zhengang,Zheng, Weihong,Li, Zhengli,&郑红花.(2013).The PI3K/Akt and ERK1/2 signaling pathways mediate the erythropoietin-modulated calcium influx in kainic acid-induced epilepsy.http://dx.doi.org/10.1097/WNR.0b013e32835ffe03.
MLA	Zheng, Honghua,et al."The PI3K/Akt and ERK1/2 signaling pathways mediate the erythropoietin-modulated calcium influx in kainic acid-induced epilepsy".http://dx.doi.org/10.1097/WNR.0b013e32835ffe03 (2013).