Distribution of Bone-Marrow-Derived Endothelial and Immune Cells in a Murine Colitis-Associated Colorectal Cancer Model

CORC > 厦门大学 > 医学院－已发表论文

	Distribution of Bone-Marrow-Derived Endothelial and Immune Cells in a Murine Colitis-Associated Colorectal Cancer Model
	Xiao, Chuan-Xing ; Wang, Huan-Huan ; Shi, Ying ; Li, Ping ; Liu, Yun-Peng ; Ren, Jian-Lin ; Guleng, Bayasi ; Xiao CX(肖传兴) ; Wang HH(王欢欢) ; Ren JL(任建林) ; 巴亚斯古楞
刊名	http://dx.doi.org/10.1371/journal.pone.0073666
	2013
关键词	TUMOR ANGIOGENESIS INTESTINAL INFLAMMATION DENDRITIC CELLS MYELOID CELLS PROGENITOR CELLS STEM-CELL T-CELLS GROWTH TOLERANCE
英文摘要	National Natural Science Foundation of China [81072013, 91229201]; Fundamental Research Funds for the Central Universities in China [2010111082]; Foundations of Health Bureau of Xiamen in China [3502Z200940010]; Inflammatory bowel disease (IBD) can lead to an increased risk of developing colorectal cancer (CRC). The aim of this study was to establish a model for combined bone marrow transplantation (BMT) and colitis-associated colorectal cancer (CAC) and to define the contribution of BM-derived cells during the inflammation associated with carcinogenesis. We established a model for BMT using green fluorescent protein (GFP) transgenic mice, followed by AOM/DSS-induced CAC, and performed confocal microscopy analysis on in vivo living tissue and frozen tumor sections. Our imaging analyses showed that GFP-positive cells extensively infiltrated the tumor stroma and that some WGA and GFP or CD31 and GFP double-positive cells were observed in the lining of tumor vessels. Flow cytometry analysis of the tumor-infiltrating cells showed that the GFP-positive CD11c+ DCs cells were one-third of the GFP+/CD11C-cells, and that half of these DCs (0.96% vs 1.02%) were GFP-positive BM-derived cells. The majority of CD4(+) T cells were GFP-negative (12.02% vs 1.9%), and we discovered a novel CD4(+) CD11c(+) DC subset (0.34% vs 1.64%). In conclusion, we defined the distribution of BM-derived endothelial cells, CD11c(+) DCs and CD4(+) T cells in tumors. This model might be useful for elucidating the diverse BM-derived cell types and functions during the progression of colitis-associated colorectal cancer.
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93491]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Xiao, Chuan-Xing,Wang, Huan-Huan,Shi, Ying,et al. Distribution of Bone-Marrow-Derived Endothelial and Immune Cells in a Murine Colitis-Associated Colorectal Cancer Model[J]. http://dx.doi.org/10.1371/journal.pone.0073666,2013.
APA	Xiao, Chuan-Xing.,Wang, Huan-Huan.,Shi, Ying.,Li, Ping.,Liu, Yun-Peng.,...&巴亚斯古楞.(2013).Distribution of Bone-Marrow-Derived Endothelial and Immune Cells in a Murine Colitis-Associated Colorectal Cancer Model.http://dx.doi.org/10.1371/journal.pone.0073666.
MLA	Xiao, Chuan-Xing,et al."Distribution of Bone-Marrow-Derived Endothelial and Immune Cells in a Murine Colitis-Associated Colorectal Cancer Model".http://dx.doi.org/10.1371/journal.pone.0073666 (2013).