Recombinant human leptin induces growth inhibition and apoptosis in human gastric cancer MGC-803 cells

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	Recombinant human leptin induces growth inhibition and apoptosis in human gastric cancer MGC-803 cells
	Wu, Na ; Wang, Yongjun ; Wang, Shengyu ; Chen, Yuqiang ; Yan, Jianghua ; Chen YQ(陈玉强) ; Yan JH(颜江华)
刊名	http://dx.doi.org/10.1007/s10238-012-0211-8
	2013
关键词	SIGNAL-TRANSDUCTION PATHWAYS PROLIFERATIVE RESPONSE CYTOCHROME-C IN-VITRO EXPRESSION RECEPTOR ACTIVATION STAT3 INVASIVENESS SURVIVIN
英文摘要	Science and Technology Innovation Fund of Xiamen City [3502Z20104014]; Science and Technology Foundation of Fujian Province [2009R10020-1]; National Natural Science Foundation of China [30973485]; The aim of this study is to investigate the effect of recombinant human leptin (rhLep) on the proliferation of human gastric cancer MGC-803 cells and its underlying mechanisms. RT-PCR was performed to identify the expression of leptin receptor (Ob-R). Cell proliferation was measured with MTT assay. DNA content and cell cycle were analyzed by flow cytometry. Apoptosis was assessed by DNA ladder assay and flow cytometry analysis using Annexin V-FITC/PI double staining. Underlying mechanisms of rhLep-induced apoptosis were evaluated by the activities of caspase-3, -8, -9, and cytochrome c release from mitochondria. Moreover, the phosphorylation of STAT3 in MGC-803 cells upon rhLep administration was detected by Western blot analysis. Our results demonstrated that two leptin receptors (Ob-Ra and Ob-Rb) were expressed in MGC-803 cells. rhLep diminished the proliferation rate of MGC-803 cells in a time- and concentration-dependent manner and induced MGC-803 cell apoptosis involving in the activation of caspase-8 and caspase-3 but not caspase-9. In addition, rhLep failed to induce cytochrome c release from mitochondria and had no effect on the activation of STAT3 in MGC-803 cells. Therefore, from these results, we concluded that rhLep significantly inhibited cell proliferation via G0/G1 phase cell cycle arrest and induced apoptosis through the extrinsic apoptotic pathway in human gastric cancer MGC-803 cells.
语种	英语
出版者	SPRINGER-VERLAG ITALIA SRL
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93458]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Wu, Na,Wang, Yongjun,Wang, Shengyu,et al. Recombinant human leptin induces growth inhibition and apoptosis in human gastric cancer MGC-803 cells[J]. http://dx.doi.org/10.1007/s10238-012-0211-8,2013.
APA	Wu, Na.,Wang, Yongjun.,Wang, Shengyu.,Chen, Yuqiang.,Yan, Jianghua.,...&颜江华.(2013).Recombinant human leptin induces growth inhibition and apoptosis in human gastric cancer MGC-803 cells.http://dx.doi.org/10.1007/s10238-012-0211-8.
MLA	Wu, Na,et al."Recombinant human leptin induces growth inhibition and apoptosis in human gastric cancer MGC-803 cells".http://dx.doi.org/10.1007/s10238-012-0211-8 (2013).