CHOP deficiency results in elevated lipopolysaccharide-induced inflammation and kidney injury | |
Esposito, Vittoria ; Grosjean, Fabrizio ; Tan, Jianming ; Huang, Liangfu ; Zhu, Libing ; Chen, Jian ; Xiong, Huabao ; Striker, Gary E. ; Zheng, Feng ; Tan JM(谭建明) ; Zheng F(郑丰) | |
刊名 | http://dx.doi.org/10.1152/ajprenal.00487.2011 |
2013 | |
关键词 | ENDOPLASMIC-RETICULUM-STRESS UNFOLDED PROTEIN RESPONSE PANCREATIC BETA-CELLS ACUTE-RENAL-FAILURE ER STRESS TRANSCRIPTION FACTOR TRANSMEMBRANE PROTEIN OXIDATIVE-STRESS EPITHELIAL-CELLS MAMMALIAN-CELLS |
英文摘要 | National Nature Science Foundation of China [81070272]; 973 Project [2012CB517601]; National Institute on Aging [5R01-AG-027628]; Genzyme Renal Innovation Grant; Italian Society of Nephrology (SIN); Esposito V, Grosjean F, Tan J, Huang L, Zhu L, Chen J, Xiong H, Striker GE, Zheng F. CHOP deficiency results in elevated lipopolysaccharide-induced inflammation and kidney injury. Am J Physiol Renal Physiol 304: F440-F450, 2013. First published December 12, 2012; doi:10.1152/ajprenal.00487.2011.-C/EBP homologous protein (CHOP) is an important mediator of endoplasmic reticulum (ER) stress-induced cell and organ injury. Here we show that lipopolysaccharide (LPS)-induced acute kidney injury (AKI) is associated with ER stress and elevated CHOP. We postulated that CHOP-/- mice would be protected against LPS-induced-AKI. Unexpectedly, while Toll-like receptor 4 (TLR4) expression levels were comparable in kidneys of CHOP-/- and wild-type (WT) mice, CHOP-/- mice developed more severe AKI after LPS injection. Furthermore, the severe kidney injury in CHOP-/- mice was associated with an exaggerated inflammatory response. Serum TNF-alpha levels were more elevated in LPS-treated CHOP-/- mice. There was a 3.5-fold higher amount of renal neutrophil infiltrates in LPS-treated CHOP-/- than in WT mice. Additionally, the kidneys of LPS-treated CHOP-/- mice had a more prominent increase in NF-kappa B activation and further upregulation of proinflammatory genes, i.e., c-x-c motif ligand 1 (CXCL-1), macrophage inflammatory protein-2 (MIP-2), and IL-6. Finally, proximal tubules, glomeruli, and podocytes isolated from CHOP-/- mice also had an exaggerated proinflammatory response to LPS. Since LPS directly increased CHOP in glomeruli and podocytes of WT mice, together these data suggest that the LPS-induced increase of CHOP in kidneys may inhibit inflammatory response in renal cells and provide protection against AKI. |
语种 | 英语 |
出版者 | AMER PHYSIOLOGICAL SOC |
内容类型 | 期刊论文 |
源URL | [http://dspace.xmu.edu.cn/handle/2288/93400] |
专题 | 医学院-已发表论文 |
推荐引用方式 GB/T 7714 | Esposito, Vittoria,Grosjean, Fabrizio,Tan, Jianming,et al. CHOP deficiency results in elevated lipopolysaccharide-induced inflammation and kidney injury[J]. http://dx.doi.org/10.1152/ajprenal.00487.2011,2013. |
APA | Esposito, Vittoria.,Grosjean, Fabrizio.,Tan, Jianming.,Huang, Liangfu.,Zhu, Libing.,...&郑丰.(2013).CHOP deficiency results in elevated lipopolysaccharide-induced inflammation and kidney injury.http://dx.doi.org/10.1152/ajprenal.00487.2011. |
MLA | Esposito, Vittoria,et al."CHOP deficiency results in elevated lipopolysaccharide-induced inflammation and kidney injury".http://dx.doi.org/10.1152/ajprenal.00487.2011 (2013). |
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