Protective effects of apocynin and allopurinol on ischemia/reperfusion-induced liver injury in mice

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	Protective effects of apocynin and allopurinol on ischemia/reperfusion-induced liver injury in mice
	Liu, Ping-Guo ; He, Song-Qing ; Zhang, Yan-Hong ; Wu, Jian ; Liu PG(刘平果)
刊名	http://dx.doi.org/10.3748/wjg.14.2832
	2008-05-14
关键词	ISCHEMIA-REPERFUSION INJURY NADPH OXIDASE OXIDATIVE STRESS SUPEROXIDE-DISMUTASE RATS NEUTROPHILS ACTIVATION APOPTOSIS DELIVERY CELLS
英文摘要	AIM: To determine the effects of allopurinol, an inhibitor of xanthine oxidase, and apocynin, an inhibitor of NADPH oxidase, on oxidant stress and liver injury caused by hepatic ischemia/reperfusion (I/R) procedure in mice. METHODS: Mice were pretreated with a xanthine oxidase inhibitor, allopurinol, or NADPH oxidase (NOX) inhibitor, apocynin before the hepatic I/R procedure. Then treated or untreated mice underwent the hepatic I/R procedure. The effects on hepatic injury and superoxide anions were determined after starting reperfusion. RESULTS: A standard warm hepatic I/R procedure led to a marked increase in superoxide anion production as indicated by a superoxide anion tracer, MCLA. At the same time, the procedure caused profound acute liver injury, as indicated by elevated serum alanine aminotransferase and tumor necrosis factor-a levels, reduced liver glutathione levels and elevated malondialdehyde contents, as well as a high apoptotic cell count. All these changes were reversed by the use of apocynin or allopurinol prior to the hepatic I/R procedure. CONCLUSION: Allopurinol and apocynin exerted protective effects on hepatic ischemia/reperfusion injury. The protection is associated with blocking the generation of superoxide anions during the hepatic I/R procedure by inhibiting xanthine oxidase and NADPH oxidase activity. (C) 2008 WJG. All rights reserved.
语种	英语
出版者	WORLD J GASTROENTERO
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93379]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Liu, Ping-Guo,He, Song-Qing,Zhang, Yan-Hong,et al. Protective effects of apocynin and allopurinol on ischemia/reperfusion-induced liver injury in mice[J]. http://dx.doi.org/10.3748/wjg.14.2832,2008.
APA	Liu, Ping-Guo,He, Song-Qing,Zhang, Yan-Hong,Wu, Jian,&刘平果.(2008).Protective effects of apocynin and allopurinol on ischemia/reperfusion-induced liver injury in mice.http://dx.doi.org/10.3748/wjg.14.2832.
MLA	Liu, Ping-Guo,et al."Protective effects of apocynin and allopurinol on ischemia/reperfusion-induced liver injury in mice".http://dx.doi.org/10.3748/wjg.14.2832 (2008).