Changes in histone acetylation during oocyte meiotic maturation in the diabetic mouse

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	Changes in histone acetylation during oocyte meiotic maturation in the diabetic mouse
	Ding, L. ; Pan, R. ; Huang, X. ; Wang, J. -X. ; Shen, Y. -T. ; Xu, L. ; Zhang, Y. ; Liu, Y. ; He, X. -Q. ; Yang, X. -J. ; Qi, Z. -Q. ; Wang, H. -L. ; Zhang Y(张业) ; Liu Y(刘洋)
刊名	http://dx.doi.org/10.1016/j.theriogenology.2012.03.026
	2012-09-01
关键词	CHROMOSOME ALIGNMENT MEIOSIS MICE DEACETYLATION CHROMATIN TRICHOSTATIN PREGNANCY MELLITUS MITOSIS FAMILY
英文摘要	National Natural Science Foundation of China [31140031]; Fundamental Research Funds for the Central Universities [2011121065]; Although there is considerable evidence that diabetes can adversely affect meiosis in mammalian oocytes, acetylation status of oocytes in a diabetic environment remains unclear. The objective was to determine acetylation or deacetylation patterns (based on immunostaining) of H3K9, H3K14, H4K5, H4K8, H4K12, and H4K16 sites at various stages during meiosis in murine oocytes from control and diabetic mice. According to quantitative real time polymerase chain reaction (qPCR), mean +/- SEM relative expression of Gcn5 (1.70 +/- 0.14 at metaphase [M]I and 1.27 +/- 0.01 at MIII, respectively), Ep300 (1.74 +/- 0.04 at MI and 1.80 +/- 0.001 at MII), and Pcaf (2.01 +/- 0.03 at MI and 1.41 +/- 0.18 at MII) mRNA in oocytes from diabetic mice were higher than those from controls (P < 0.05), whereas there was no difference (P > 0.05) during the germinal vesicle (GV) stage between the two groups (1.23 +/- 0.04 for Gcn5, 0.82 +/- 0.06 for Ep300, and 0.80 +/- 0.07 for Pcaf). Conversely, relative mRNA expression concentrations of Hdac1, Hdac2, Hdac3, Sirt1 and Sirt2 during the germinal vesicle stage were lower in oocytes of diabetic mice (0.24 +/- 0.03 for Hdac1, 0.11 +/- 0.001 for Hdac2, 0.31 +/- 0.03 for Hdac3, 0.28 +/- 0.02 for Sirt1, and 0.55 +/- 0.02 for Sirt2; P < 0.05). Similarly, the expression concentrations of these genes at the MI stage were lower in oocytes from diabetic mice (0.79 +/- 0.12 for Hdac1, 0.72 +/- 0.001 for Hdac2, 0.02 +/- 0.001 for Sirt1, and 0.84 +/- 0.08 for Sirt2; P < 0.05). Their expression concentrations at the ME stage were also lower in oocytes from diabetic mice (0.46 +/- 0.03 for Hdac1, 0.93 +/- 0.01 for Hdac2, 0.56 +/- 0.01 for Hdac3, 0.01 +/- 0.002 for Sirt1, and 0.84 +/- 0.04 for Sirt2; P < 0.05). At the MI stage, however, there was no difference in the expression of Hdac3 between the two groups of oocytes (0.96 +/- 0.03; P > 0.05). Taken together, diabetes altered the intracellular histone modification system, which may have contributed to changes in histone acetylation, and may be involved in the compromised maturation rate of oocytes in diabetic humans. (C) 2012 Elsevier Inc. All rights reserved.
语种	英语
出版者	THERIOGENOLOGY
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93332]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Ding, L.,Pan, R.,Huang, X.,et al. Changes in histone acetylation during oocyte meiotic maturation in the diabetic mouse[J]. http://dx.doi.org/10.1016/j.theriogenology.2012.03.026,2012.
APA	Ding, L..,Pan, R..,Huang, X..,Wang, J. -X..,Shen, Y. -T..,...&刘洋.(2012).Changes in histone acetylation during oocyte meiotic maturation in the diabetic mouse.http://dx.doi.org/10.1016/j.theriogenology.2012.03.026.
MLA	Ding, L.,et al."Changes in histone acetylation during oocyte meiotic maturation in the diabetic mouse".http://dx.doi.org/10.1016/j.theriogenology.2012.03.026 (2012).