Notch1 signaling inhibits growth of EC109 esophageal carcinoma cells through downmodulation of HPV18 E6/E7 gene expression

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	Notch1 signaling inhibits growth of EC109 esophageal carcinoma cells through downmodulation of HPV18 E6/E7 gene expression
	Zhang, Ke-jie ; Lu, Quan-yi ; Niu, Xiao-qing ; Zhang, Peng ; Zhao, Jiang-ning ; Wang, Zhao ; Hu, Jia-sheng ; Li, Pu ; Liu, Wen-li ; Zhang KJ(张可杰) ; Lu QY(鹿全意) ; Niu XQ(牛小青) ; Zhang P(张鹏) ; Zhao JN(赵江宁) ; Wang Z(王昭) ; Hu JS(胡嘉升)
刊名	http://dx.doi.org/10.1038/aps.2008.16
	2009-02
关键词	CANCER CELLS P53 DIFFERENTIATION HEMATOPOIESIS ACTIVATION ARREST ROLES
英文摘要	National Natural Science Foundation of China [30570773]; Aim: To investigate the role of the Notch1 signaling pathway in growth arrest of an esophageal carcinoma cell line (EC109) in vitro and the mechanism involved. Methods: An intracellular domain of Notch1 (ICN) was transfected into cultured EC109 cells by lipofectamine transfection. Subsequently, the proliferation of the transfected cells was measured by an MTT assay. Cell cycle distribution was analyzed by flow cytometry. Human papillomavirus type 18 (HPV18) E6/E7 mRNA expression was detected by RT-PCR, and p53 protein expression was detected by Western blot. Results: Activation of Notch1 signaling resulted in inhibition of EC109 cell proliferation with the induction of G2/M arrest, downmodulation of HPV18 E6/E7 gene expression, and upregulation of p53 expression. Conclusion: Repression of HPV18 E6/E7 expression by Notch1 signaling results in the activation of p53-mediated pathways with concomitant growth suppression of HPV18-positive EC109 cells.
语种	英语
出版者	ACTA PHARMACOL SIN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93154]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Zhang, Ke-jie,Lu, Quan-yi,Niu, Xiao-qing,et al. Notch1 signaling inhibits growth of EC109 esophageal carcinoma cells through downmodulation of HPV18 E6/E7 gene expression[J]. http://dx.doi.org/10.1038/aps.2008.16,2009.
APA	Zhang, Ke-jie.,Lu, Quan-yi.,Niu, Xiao-qing.,Zhang, Peng.,Zhao, Jiang-ning.,...&胡嘉升.(2009).Notch1 signaling inhibits growth of EC109 esophageal carcinoma cells through downmodulation of HPV18 E6/E7 gene expression.http://dx.doi.org/10.1038/aps.2008.16.
MLA	Zhang, Ke-jie,et al."Notch1 signaling inhibits growth of EC109 esophageal carcinoma cells through downmodulation of HPV18 E6/E7 gene expression".http://dx.doi.org/10.1038/aps.2008.16 (2009).