hSHIP induces S-phase arrest and growth inhibition in cervical cancer HeLa cells

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	hSHIP induces S-phase arrest and growth inhibition in cervical cancer HeLa cells
	He, K. X. ; He, J. ; Wang, S. Y. ; Yan, J. H. ; Yan JH(颜江华)
刊名	http://dx.doi.org/10.1016/S1673-8527(09)60043-6
	2010-04
关键词	INOSITOL PHOSPHATASE SHIP CHRONIC MYELOGENOUS LEUKEMIA TARGETED DISRUPTION FACTOR-RECEPTOR HIGH-FREQUENCY PIK3CA GENE ACTIVATION PATHWAY MICE MUTATIONS
英文摘要	985 Research Foundation of Xiamen University; Research Foundation of Cancer Research Center, Xiamen University; hSHIP, a human SH2-containing inositol-5-phosphatase, acts as a negative regulator of proliferation and survival in hematopoietic cells. Therefore, hSHIP may play a crucial role in suppression of cervical cancer HeLa cells. In this study, pcDNA3.1-hSHIP-GFP plasmid was constructed and transfected into HeLa cells with Lipofectamine2000, stably transfected HeLa cells were established and their responses were investigated by Flow cytometry, MTT, tumorigenicity in nude mice, RT-PCR and ELISA assays. The results showed that the expression of hSHIP significantly induced S-phase arrest, cell growth inhibition, and down-regulation of Akt1/2 mRNA and p-Akt in HeLa cells. Our study supports an important role for hSHIP in suppression of cervical cancer HeLa cells, which may prove to be a novel therapeutic option for non-hematopoietic cancers.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/71525]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	He, K. X.,He, J.,Wang, S. Y.,et al. hSHIP induces S-phase arrest and growth inhibition in cervical cancer HeLa cells[J]. http://dx.doi.org/10.1016/S1673-8527(09)60043-6,2010.
APA	He, K. X.,He, J.,Wang, S. Y.,Yan, J. H.,&颜江华.(2010).hSHIP induces S-phase arrest and growth inhibition in cervical cancer HeLa cells.http://dx.doi.org/10.1016/S1673-8527(09)60043-6.
MLA	He, K. X.,et al."hSHIP induces S-phase arrest and growth inhibition in cervical cancer HeLa cells".http://dx.doi.org/10.1016/S1673-8527(09)60043-6 (2010).