Optimized capillary based microarray used for DNA analysis | |
Chen, Ho ; Ge, Likai ; Zheng, Zezhi ; Qu, Xiangmeng ; Lan, Xiaoling ; Chen H(陈红) | |
2013 | |
关键词 | Contamination DNA Drop formation Fluidic devices Microarrays |
英文摘要 | Conference Name:8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013. Conference Address: Suzhou, China. Time:April 7, 2013 - April 10, 2013.; Institute of Electrical and Electronics Engineers (IEEE); IEEE Nanotechnology Council (NTC); Peking University; Soochow University; Chinese Academy of Sciences; Microarray is an very useful tool for nucleic acid detection, but the procedure is labor intensive and time consuming. Microfluidic chip-based microarray saves time with better performance, whlie the low spot density and probe number limit its applications in many related fields. To develop high performance microarray with higher spot density and probe number, a method is reported here to prepare microarray in a capillary by generating probe droplets array. The probes in droplets are immobilized onto the inner wall of the capillary to form an one-dimensional probe array, and then a sample solution is introduced to hybridize with the probe array. This paper demonstrated the droplets array, probe array and hybridized spots array which were generated inside the capillary, then the cross contamination caused by the generation of droplets array was evaluated. Data showed that the cross contamination was only a very small portion. It can be regarded as the background and eliminated by subtracting. ? 2013 IEEE. |
语种 | 英语 |
出处 | http://dx.doi.org/10.1109/NEMS.2013.6559738 |
出版者 | IEEE Computer Society |
内容类型 | 其他 |
源URL | [http://dspace.xmu.edu.cn/handle/2288/86638] |
专题 | 信息技术-会议论文 |
推荐引用方式 GB/T 7714 | Chen, Ho,Ge, Likai,Zheng, Zezhi,et al. Optimized capillary based microarray used for DNA analysis. 2013-01-01. |
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