Two-dimensional capillary electrophoresis involving capillary isoelectric focusing and capillary zone electrophoresis

	Two-dimensional capillary electrophoresis involving capillary isoelectric focusing and capillary zone electrophoresis
	Yang, C; Zhang, LY; Liu, HC; Zhang, WB; Zhang, YK
刊名	journal of chromatography a
	2003-11-07
卷号	1018 期号:1 页码:97-103
关键词	two-dimensional capillary electrophoresis capillary isoelectric focusing capillary electrophoresis dialysis interface electroosmotic flow
产权排序	1;1
英文摘要	capillary isoelectric focusing (cief) and capillary zone electrophoresis (cze) was on-line hyphenated by a dialysis interface to achieve a 2d capillary electrophoresis (ce) system. the system was used with just one high-voltage power supply and three electrodes (one cathode shared by the two dimensions). the focused zone in the first dimension (i.e. the cief) was driven to the dialysis interface by electroosmotic flow (eof), besides chemical mobilization from the first anode to the shared cathode. and then in the second dimension (i.e. the cze), the separated zone was further separated and driven by an inverted eof, which originated from the charged layer of a cationic surfactant adsorbed onto the inner wall of the capillary. finally, a solution of ribonuclease was rapidly separated to assess the feasibility of the two-dimensional ce implement. (c) 2003 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	basic-proteins ; separation ; chromatography ; peptides ; isotachophoresis ; interface ; system ; column ; microdialysis ; configuration
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000185856700010
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/83487]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Yang, C,Zhang, LY,Liu, HC,et al. Two-dimensional capillary electrophoresis involving capillary isoelectric focusing and capillary zone electrophoresis[J]. journal of chromatography a,2003,1018(1):97-103.
APA	Yang, C,Zhang, LY,Liu, HC,Zhang, WB,&Zhang, YK.(2003).Two-dimensional capillary electrophoresis involving capillary isoelectric focusing and capillary zone electrophoresis.journal of chromatography a,1018(1),97-103.
MLA	Yang, C,et al."Two-dimensional capillary electrophoresis involving capillary isoelectric focusing and capillary zone electrophoresis".journal of chromatography a 1018.1(2003):97-103.