Effect of 3D microgroove surface topography on plasma and cellular fibronectin of human gingival fibroblasts

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	Effect of 3D microgroove surface topography on plasma and cellular fibronectin of human gingival fibroblasts
	Lai, Yingzhen ; Chen, Jiang ; Zhang, Tao ; Gu, Dandan ; Zhang, Chunquan ; Li, Zuanfang ; Lin, Shan ; Fu, Xiaoming ; Schultze-Mosgau, Stefan ; Gu DD(谷丹丹)
刊名	http://dx.doi.org/10.1016/j.jdent.2013.08.004
	2013
关键词	PROTEIN ADSORPTION EXTRACELLULAR-MATRIX ALBUMIN ADSORPTION ADHESION BEHAVIOR WETTABILITY DYNAMICS IMPLANT MECHANOTRANSDUCTION HYDROXYAPATITE
英文摘要	Science and Technology Project of Fujian Province [2010H6009]; Provincial Development and Reform Commission (NDRC) project [[2010]1002]; Objectives: Fibronectin (FN), an extracellular matrix (ECM) glycoprotein, is a key factor in the compatibility of dental implant materials. Our objective was to determine the optimal dimensions of microgrooves in the transmucosal part of a dental implant, for optimal absorption of plasma FN and expression of cellular FN by human gingival fibroblasts (HGFs). Methods: Microgroove titanium surfaces were fabricated by photolithography with parallel grooves: 15 mu m, 30 mu m, or 60 mu m in width and 5 mu m or 10 mu m in depth. Smooth titanium surfaces were used as controls. Surface hydrophilicity, plasma FN adsorption and cellular FN expression by HGFs were measured for both microgroove and control samples. Results: We found that narrower and deeper microgrooves amplified surface hydrophobicity. A 15-mu m wide microgroove was the most hydrophobic surface and a 60-mu m wide microgroove was the most hydrophilic. The latter had more expression of cellular FN than any other surface, but less absorption of plasma FN than 15-mu m wide microgrooves. Variation in microgroove depth did not appear to effect FN absorption or expression unless the groove was narrow (similar to 15 or 30 mu m). In those instances, the shallower depths resulted in greater expression of cellular FN. Conclusions: Our microgrooves improved expression of cellular FN, which functionally compensated for plasma FN. A microgroove width of 60 mu m and depth of 5 or 10 mu m appears to be optimal for the transmucosal part of the dental implant. (C) 2013 Elsevier Ltd. All rights reserved.
语种	英语
出版者	ELSEVIER SCI LTD
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/91807]
专题	物理技术－已发表论文
推荐引用方式 GB/T 7714	Lai, Yingzhen,Chen, Jiang,Zhang, Tao,et al. Effect of 3D microgroove surface topography on plasma and cellular fibronectin of human gingival fibroblasts[J]. http://dx.doi.org/10.1016/j.jdent.2013.08.004,2013.
APA	Lai, Yingzhen.,Chen, Jiang.,Zhang, Tao.,Gu, Dandan.,Zhang, Chunquan.,...&谷丹丹.(2013).Effect of 3D microgroove surface topography on plasma and cellular fibronectin of human gingival fibroblasts.http://dx.doi.org/10.1016/j.jdent.2013.08.004.
MLA	Lai, Yingzhen,et al."Effect of 3D microgroove surface topography on plasma and cellular fibronectin of human gingival fibroblasts".http://dx.doi.org/10.1016/j.jdent.2013.08.004 (2013).