Microfluidic droplet-array liquid-liquid chromatography based on droplet trapping technique

CORC > 厦门大学 > 物理技术－已发表论文

	Microfluidic droplet-array liquid-liquid chromatography based on droplet trapping technique
	Zhu, Ying ; Chen, Hong ; Du, Guan-Sheng ; Fang, Qun ; Chen H(陈宏)
刊名	http://dx.doi.org/10.1039/c2lc40573c
	2012
关键词	POLYMERASE-CHAIN-REACTION INJECTION SYSTEM SINGLE CELLS EXTRACTION CHIP FLOW TECHNOLOGY INTERFACE COLUMNS DEVICES
英文摘要	National Natural Science Foundation of China [20825517, 20890020, 21105089]; We describe the first realization of liquid chromatographic separation in a droplet-based microfluidic system and develop a novel mode for microchip-based chromatography named as droplet-array liquid-liquid chromatography. In this system, two arrays of picoliter-scale droplets immobilized on both sidewalls of a microchannel with droplet trapping technique served as the stationary phase in chromatographic separation, while the other immiscible phase flowing in the microchannel served as the mobile phase. The chromatographic separation was achieved on the basis of multiple extraction and elution of analytes between the droplet array stationary phase and the mobile phase. The proof-of-concept study of the droplet-array LC system was performed in the separation of fluoranthene and benzo[b]fluoranthene. Under the optimum conditions, the two analytes were separated within 26 min with separation efficiencies of 112 mu m and 119 mu m plate height, respectively. The advantages of the present system include simple structure, low driving pressure, and relatively high sample capacity. It can also provide a useful platform for LC theory study and educational purposes by allowing the researchers and students to directly "see" the continuous extraction and elution process of a chromatographic separation.
语种	英语
出版者	LAB CHIP
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/91554]
专题	物理技术－已发表论文
推荐引用方式 GB/T 7714	Zhu, Ying,Chen, Hong,Du, Guan-Sheng,et al. Microfluidic droplet-array liquid-liquid chromatography based on droplet trapping technique[J]. http://dx.doi.org/10.1039/c2lc40573c,2012.
APA	Zhu, Ying,Chen, Hong,Du, Guan-Sheng,Fang, Qun,&陈宏.(2012).Microfluidic droplet-array liquid-liquid chromatography based on droplet trapping technique.http://dx.doi.org/10.1039/c2lc40573c.
MLA	Zhu, Ying,et al."Microfluidic droplet-array liquid-liquid chromatography based on droplet trapping technique".http://dx.doi.org/10.1039/c2lc40573c (2012).