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	PKD3 is required for prostratin-activated HIV-1 transcription
	Zhu, Ying ; Ding, Feng ; Juventus, Aweya Jude ; Zhou, Nan ; Huan, Zeng ; Wu, Shanwen ; Wang, Yu ; Tong, Xinzhu ; Chen, Ruichuan ; Liu, Runzhong ; Ding F(丁凤) ; Chen RC(陈瑞川) ; Liu RZ(刘润忠)
	2011
关键词	Enzymes Genes Phosphorylation
英文摘要	Conference Name:2011 IEEE International Symposium on IT in Medicine and Education, ITME 2011. Conference Address: Guangzhou, China. Time:December 9, 2011 - December 11, 2011.; IEEE Sapporo Section; Lanzhou University (LZU); Henan University of Technology (HAUT); Wuhan University of Technology (WHUT); East China Normal University (ECNU); Prostratin has been shown to promote transcriptional activation of HIV-1 provirus, however, the underlying mechanism is not fully understood. By using HeLa cells with a stably integrated HIV-LTR-Luciferase gene as a reporter system, we investigated the role of protein kinase D (PKD) in HIV-1 activation by prostratin treatment. Knocking-down of PKD3, but not PKD1 or PKD2, inhibited both the basal and prostratin-induced HIV-LTR-Luciferase expression. Moreover, prostratin triggered PKD3 activation by inducing the phosphorylation of its activation loop. Furthermore, the overexpression of constitutively active form of PKD3, but not the wild type or kinase dead form of PKD3, augmented HIV-LTR-Luciferase expression. These results suggest that activated PKD3 is critical for prostratin stimulating HIV-LTR transcription. ? 2011 IEEE.
语种	英语
出处	http://dx.doi.org/10.1109/ITiME.2011.6132184
出版者	IEEE Computer Society
内容类型	其他
源URL	[http://dspace.xmu.edu.cn/handle/2288/85892]
专题	生命科学－会议论文
推荐引用方式 GB/T 7714	Zhu, Ying,Ding, Feng,Juventus, Aweya Jude,et al. PKD3 is required for prostratin-activated HIV-1 transcription. 2011-01-01.

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