| 福建茶区小贯小绿叶蝉rDNA序列分析及形态再描述; r DNA sequence analysis and morphological redescription of Empoasca onukii from the tea growing area of Fujian | |
| 章旭日 ; 潘杰 ; Tukayo ; Meks ; 余良 ; 方文珍 ; 罗大民 | |
| 2015-10-25 | |
| 关键词 | 小贯小绿叶蝉 形态 rDNA ITS2 Empoasca onukii morphology rDNA ITS2 |
| 英文摘要 | 重新描述了小贯小绿叶蝉的形态结构,尤其是头部色斑、翅脉、腹部内突、下生殖板及其刚毛着生位置,并增加了足部(刺毛列)特征描述。通过PCr技术克隆得到该物种的r dnA序列,包括部分18S(1 843bP)和28S序列(667 bP)以及完整的5.8S(155 bP)、ITS1(3 114 bP)和ITS2序列(1 008 bP)。序列分析表明,18S,5.8S,28S与其他物种间具有90%~95%的序列一致性;而ITS1和ITS2序列变异性非常大。碱基组成比率分析显示ITS1以及ITS2具有AT偏好性,前者A+T占66.0%,后者占65.1%。与亲缘种的比较分析显示ITS1和ITS2具有丰富的多态位点,并且ITS2更适用于近缘种的分子鉴定。; The morphological structures of Empoasca onukii,the cephalic splashes,vein,abdominal apodemes,subgenital plate and the arrangement of its bristles,and the characteristics of legs were redescribed,especially the arrangement of spines were added.Ribosomal DNA( r DNA) including partial of 18S( 1 843 bp),28S( 667 bp) and complete 5.8S( 155 bp),ITS1( 3 114 bp),ITS2( 1 008 bp) were isolated by PCR technology.Sequence analysis indicated that 18 S,5.8S and 28 S had high sequence identity( 90%- 95%) with other insects; however,ITS1 and ITS2 showed much variability.The analysis of base composition showed that ITS1 and ITS2 were more preferences for AT( contained 66.0% and 65.1% A + T,respectively).Comparison with the phylogenetic species revealed that ITS1 and ITS2 from E.onukii had rich polymorphic loci and ITS2 was more suitable for molecular identification of closely related species.; 国家自然科学基金(81171595) |
| 语种 | zh_CN |
| 内容类型 | 期刊论文 |
| 源URL | [http://dspace.xmu.edu.cn/handle/2288/119308]  |
| 专题 | 生命科学－已发表论文 |
| 推荐引用方式 GB/T 7714 | 章旭日,潘杰,Tukayo,等. 福建茶区小贯小绿叶蝉rDNA序列分析及形态再描述, r DNA sequence analysis and morphological redescription of Empoasca onukii from the tea growing area of Fujian[J],2015. |
| APA | 章旭日.,潘杰.,Tukayo.,Meks.,余良.,...&罗大民.(2015).福建茶区小贯小绿叶蝉rDNA序列分析及形态再描述.. |
| MLA | 章旭日,et al."福建茶区小贯小绿叶蝉rDNA序列分析及形态再描述".(2015). |
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