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	斑节对虾两个野生亲虾种群遗传多样性; Genetic diversity of two wild Penaeus monodon broodstock populations
	谭树华 ; 王桂忠 ; 李少菁
	2011
关键词	斑节对虾 RAPD 16SrRNA 遗传多样性 遗传分化 Penaeus monodon RAPD 16S rRNA genetic diversity genetic differentiation
英文摘要	采用rAPd和MTdnA16SrrnA基因序列分析方法对海南和马来西亚斑节对虾(PEnAEuS MOnOdOn)种群进行了遗传多样性和遗传分化研究。结果表明:15个rAPd随机引物共检测到82个位点,海南和马来西亚种群的多态位点比例分别为75.90%和76.83%,杂合度分别为0.199和0.218,遗传多样性指数分别为0.276和0.288,种群间的遗传距离为0.015;16SrrnA基因检测的种内遗传变异较低,马来西亚和海南种群的核苷酸多样性分别为0.011和0,种群之间的遗传距离为0.008;马来西亚种群比海南种群的遗传变异水平要高得多,且海南种群可能起源于马来西亚种群;进行遗传选育时可考虑引进马来西亚亲虾作为奠基群体。; By using RAPD and mtDNA 16S rRNA gene sequence analysis,this paper studied the genetic diversity and differentiation of two Penaeus monodon wild broodstock populations collected from Hainan and Malaysia.Using 15 random primers,a total of 82 RAPD loci were detected.For the Hainan and Malaysia populations,the proportion of polymorphic loci was 75.90% and 76.83%,heterozygosity was 0.199 and 0.218,and genetic diversity index was 0.276 and 0.288,respectively,and the genetic distance between the two populations was 0.015.The genetic variation revealed by 16S rRNA gene sequence analysis was low.The nucleotide diversity of the Hainan and Malaysia populations was 0.011 and 0,respectively,and the genetic distance between the two populations was 0.008.Phylogeographic pattern analysis indicated that Malaysia population had a higher genetic diversity than Hainan population,and the Hainan population could be derived from Malaysia population.Therefore,the broodstock imported from Malaysia could be used as the founder in genetic breeding programs.; 国家自然科学基金项目(30471322);湖南省教育厅资助科研项目(08B024)资助
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/119047]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	谭树华,王桂忠,李少菁. 斑节对虾两个野生亲虾种群遗传多样性, Genetic diversity of two wild Penaeus monodon broodstock populations[J],2011.
APA	谭树华,王桂忠,&李少菁.(2011).斑节对虾两个野生亲虾种群遗传多样性..
MLA	谭树华,et al."斑节对虾两个野生亲虾种群遗传多样性".(2011).

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