Inactivation kinetics of formaldehyde on N-acetyl-beta-d-glucosaminidase from Nile tilapia (Oreochromis niloticus)

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	Inactivation kinetics of formaldehyde on N-acetyl-beta-d-glucosaminidase from Nile tilapia (Oreochromis niloticus)
	Zhang, Wei-Ni ; Bai, Ding-Ping ; Lin, Xin-Yu ; Chen, Qing-Xi ; Huang, Xiao-Hong ; Huang, Yi-Fan ; Zhang WN(张伟妮) ; Chen QX(陈清西)
刊名	http://dx.doi.org/10.1007/s10695-013-9866-7
	2014
关键词	INHIBITORY KINETICS HEXOSAMINIDASE EXPRESSION VANNAMEI EGGS
英文摘要	Science and Technology Major Project of Fujian Province [2012N0002]; Science and Technology Project of Fujian Provincial Committee of Education [JK2012013]; Formaldehyde is a widely used sanitizer in aquaculture in China, while the appropriate concentration is not available to be used effectively and without damage to tilapia much less to its reproductive function. N-acetyl-beta-d-glucosaminidase (EC 3.2.1.52, NAGase), hydrolyzing the oligomers of N-acetyl-beta-d-glucosamine into monomer, is proved to be correlated with reproduction of male animals. In this paper, NAGase from spermary of tilapia was chosen as the material to study the effects of formaldehyde on its activity in order to further investigate the effects of formaldehyde use on tilapia reproduction. The results showed the relationship between the residual enzyme activity and the concentration of formaldehyde was concentration dependent, and the IC50 value was estimated to be 3.2 +/- A 0.1 %. Appropriate concentration of formaldehyde leaded to competitive reversible inhibition on tilapia NAGase. Moreover, formaldehyde could reduce the thermal and pH stability of the enzyme. The inactivation kinetics of formaldehyde on the enzyme was studied using the kinetic method of substrate reaction. The inactivation model was setup, and the rate constants were determined. The results showed that the inactivation of formaldehyde on tilapia NAGase was a slow, reversible reaction with partially residual activity. The results will give some basis to determine the concentration of formaldehyde used in tilapia culture.
语种	英语
出版者	SPRINGER
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90810]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Zhang, Wei-Ni,Bai, Ding-Ping,Lin, Xin-Yu,et al. Inactivation kinetics of formaldehyde on N-acetyl-beta-d-glucosaminidase from Nile tilapia (Oreochromis niloticus)[J]. http://dx.doi.org/10.1007/s10695-013-9866-7,2014.
APA	Zhang, Wei-Ni.,Bai, Ding-Ping.,Lin, Xin-Yu.,Chen, Qing-Xi.,Huang, Xiao-Hong.,...&陈清西.(2014).Inactivation kinetics of formaldehyde on N-acetyl-beta-d-glucosaminidase from Nile tilapia (Oreochromis niloticus).http://dx.doi.org/10.1007/s10695-013-9866-7.
MLA	Zhang, Wei-Ni,et al."Inactivation kinetics of formaldehyde on N-acetyl-beta-d-glucosaminidase from Nile tilapia (Oreochromis niloticus)".http://dx.doi.org/10.1007/s10695-013-9866-7 (2014).