Vitellogenin of Fujian oyster, Crassostrea angulata: Synthesized in the ovary and controlled by estradiol-17 beta

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	Vitellogenin of Fujian oyster, Crassostrea angulata: Synthesized in the ovary and controlled by estradiol-17 beta
	Ni, Jianbin ; Zeng, Zhen ; Kong, Dezheng ; Hou, Lin ; Huang, Heqing ; Ke, Caihuan ; Huang HQ(黄河清) ; Ke CH(柯才焕)
刊名	http://dx.doi.org/10.1016/j.ygcen.2014.03.034
	2014-06-01
关键词	SYDNEY ROCK OYSTER MUSSEL ELLIPTIO-COMPLANATA HALIOTIS-DISCUS-HANNAI ESTROGEN-RECEPTOR GENE PACIFIC OYSTER ENDOCRINE DISRUPTION MYTILUS-EDULIS MOLECULAR CHARACTERIZATION PATINOPECTEN-YESSOENSIS SACCOSTREA-GLOMERATA
英文摘要	National Basic Research Program of China [2010CB126403]; Program for Changjiang Scholars and Innovative Research Team in the Xiamen University [IRT0941]; Earmarked Fund for Modern Agro-industry Technology Research System [CARS-48]; Programme of Introducing Talents of Discipline to Universities [B07034]; In this study, we cloned a full-length cDNA encoding vitellogenin (Vg) in the Fujian oyster Crassostrea angulata. The complete Vg cDNA consists of 5160 nucleotides with a long open reading frame encoding 1641 amino acid residues. The deduced amino acid sequence shared high similarity with the Vgs of other mollusc, fish, nematode and arthropod species, particularly in the N-terminal region. We analyzed the spatiotemporal expression of caVg transcripts by Real-time Quantitative PCR. In common with other mollusc Vgs, the caVg gene was expressed primarily in the ovary, and the levels were 348 and 177 times higher in maturation and ripeness stages (P < 0.01), respectively, than in the partially spent stage. There was negligible expression in male oysters. In situ hybridization analysis further localized caVg mRNA to the follicle cells (also named auxiliary cells) surrounding the oocytes in the ovary. Moreover, in vivo waterborne exposure experiments in early gametogenesis oysters showed that estradiol-17 beta (E-2) administration resulted in a significant increase in caVg mRNA expression. We conclude that caVg is synthesized in the follicle cell surrounding the vitellogenic oocyte in C. angulata, and directly passed to oocytes through the extracellular space without mediation through hemolymph. Also, we hypothesize that this process is mediated by E-2 in a dose dependent. (C) 2014 Elsevier Inc. All rights reserved.
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90773]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Ni, Jianbin,Zeng, Zhen,Kong, Dezheng,et al. Vitellogenin of Fujian oyster, Crassostrea angulata: Synthesized in the ovary and controlled by estradiol-17 beta[J]. http://dx.doi.org/10.1016/j.ygcen.2014.03.034,2014.
APA	Ni, Jianbin.,Zeng, Zhen.,Kong, Dezheng.,Hou, Lin.,Huang, Heqing.,...&柯才焕.(2014).Vitellogenin of Fujian oyster, Crassostrea angulata: Synthesized in the ovary and controlled by estradiol-17 beta.http://dx.doi.org/10.1016/j.ygcen.2014.03.034.
MLA	Ni, Jianbin,et al."Vitellogenin of Fujian oyster, Crassostrea angulata: Synthesized in the ovary and controlled by estradiol-17 beta".http://dx.doi.org/10.1016/j.ygcen.2014.03.034 (2014).