Conformational Plasticity of the 2A Proteinase from Enterovirus 71

CORC > 厦门大学 > 生命科学－已发表论文

	Conformational Plasticity of the 2A Proteinase from Enterovirus 71
	Cai, Qixu ; Yameen, Muhammad ; Liu, Weihua ; Gao, Zhenting ; Li, Yaozong ; Peng, Xuanjia ; Cai, Yaxian ; Wu, Caiming ; Zheng, Qian ; Li, Jian ; Lin, Tianwei ; Li J(李剑) ; Lin TW(林天伟)
刊名	http://dx.doi.org/10.1128/JVI.03541-12
	2013
关键词	PORE COMPLEX COMPOSITION VIRUS 3C PROTEASE POLIOVIRUS INFECTION TYPE-71 INFECTIONS NMR SYSTEM DISEASE FOOT EPIDEMIC CRYSTALLOGRAPHY POLIOMYELITIS
英文摘要	National Basic Research Program of China (973 program) [2012CB724500]; Open Research Fund of the State Key Laboratory of Cellular Stress Biology, Xiamen University [SKLCSB2012KF003]; 111 Project of Education of China [B06016]; The 2A proteinase (2A(pro)) is an enterovirally encoded cysteine protease that plays essential roles in both the processing of viral precursor polyprotein and the hijacking of host cell translation and other processes in the virus life cycle. Crystallographic studies of 2A(pro) from enterovirus 71 (EV71) and its interaction with the substrate are reported here. EV71 2A(pro) was comprised of an N-terminal domain of a four-stranded antiparallel beta sheet and a C-terminal domain of a six-stranded antiparallel beta barrel with a tightly bound zinc atom. Unlike in other 2A(pro) structures, there is an open cleft across the surface of the protein in an open conformation. As demonstrated by the crystallographic studies and modeling of the complex structure, the open cleft could be fitted with the substrate. On comparison 2A(pro) of EV71 to those of the human rhinovirus 2 and coxsackievirus B4, the open conformation could be closed with a hinge motion in the bII2 and cII beta strands. This was supported by molecular dynamic simulation. The structural variation among different 2A(pro) structures indicates a conformational flexibility in the substrate-binding cleft. The open structure provides an accessible framework for the design and development of therapeutics against the viral target.
语种	英语
出版者	AMER SOC MICROBIOLOGY
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90707]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Cai, Qixu,Yameen, Muhammad,Liu, Weihua,et al. Conformational Plasticity of the 2A Proteinase from Enterovirus 71[J]. http://dx.doi.org/10.1128/JVI.03541-12,2013.
APA	Cai, Qixu.,Yameen, Muhammad.,Liu, Weihua.,Gao, Zhenting.,Li, Yaozong.,...&林天伟.(2013).Conformational Plasticity of the 2A Proteinase from Enterovirus 71.http://dx.doi.org/10.1128/JVI.03541-12.
MLA	Cai, Qixu,et al."Conformational Plasticity of the 2A Proteinase from Enterovirus 71".http://dx.doi.org/10.1128/JVI.03541-12 (2013).