A protein engineering of Bacillus thuringiensis delta-endotoxin by conjugating with 4 ''-O-succinoyl abamectin

CORC > 厦门大学 > 生命科学－已发表论文

	A protein engineering of Bacillus thuringiensis delta-endotoxin by conjugating with 4 ''-O-succinoyl abamectin
	Pan, Zhi-Zhen ; Zhu, Yu-Jing ; Chen, Zheng ; Ruan, Chuan-Qing ; Xu, Lian ; Chen, Qing-Xi ; Liu, Bo ; Zhu YJ(朱育菁) ; Chen QX(陈清西)
刊名	http://dx.doi.org/10.1016/j.ijbiomac.2013.08.044
	2013
关键词	BRUSH-BORDER MEMBRANE BUTTERFLY PIERIS-BRASSICAE BIOCIDE GCSC-BTA MANDUCA-SEXTA INSECT RESISTANCE CRY1A TOXINS BINDING CADHERIN VESICLES RECEPTOR
英文摘要	China Natural Science Foundation of Nation [31371999]; National Science and Technology Project of 'the Twelfth Five-Year-Plan' for the Rural Development in China [2011AA10A203]; Youth science and technology innovation fund project in Fujian Province [2011J05053]; Fujian academy of agricultural sciences for Young Scientists [2011QB-20]; Conjugation of Bacillus thuringiensis delta-endotoxin (Bt toxin) with other toxins for insect pest control has been proposed as a new efficient strategy with increasing insecticidal toxicity and target range and delay the onset of insect resistance. A modified method was investigated by conjugating Bt toxin with 4 ''-O-succinoyl abamectin to form a new biocide which was named as BtA. 'Zero-length' cross-linker EDC in combination with NHS activated 4 ''-O-succinoyl abamectin and extended half-life period of active intermediate for binding to Bt toxin. The dissociation constant for 4 ''-O-succinoyl abamectin binding to Bt toxin was 6.44 mu M by fluorescence quenching analysis. BtA showed a higher insecticidal toxicity against Plutella xylostella, while the relative-toxicity multiple of BtA to Bt toxin was calculated as 5.6. The interaction between Bt toxins with their receptors played a key role in toxicity of Bt toxins. The binding analysis showed the dissociation rate for the binding of BtA to its receptors (7.495 x 10(-3) S-1) was twice slower than that of Bt toxin (1.695 x 10(-2) S-1). The relative dissociation constant of BtA to Bt toxin was only 29% for the binding to the receptors. These results demonstrated that BtA bound to the receptor in BBMV with significantly higher affinity compared with Bt toxin. (C) 2013 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90653]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Pan, Zhi-Zhen,Zhu, Yu-Jing,Chen, Zheng,et al. A protein engineering of Bacillus thuringiensis delta-endotoxin by conjugating with 4 ''-O-succinoyl abamectin[J]. http://dx.doi.org/10.1016/j.ijbiomac.2013.08.044,2013.
APA	Pan, Zhi-Zhen.,Zhu, Yu-Jing.,Chen, Zheng.,Ruan, Chuan-Qing.,Xu, Lian.,...&陈清西.(2013).A protein engineering of Bacillus thuringiensis delta-endotoxin by conjugating with 4 ''-O-succinoyl abamectin.http://dx.doi.org/10.1016/j.ijbiomac.2013.08.044.
MLA	Pan, Zhi-Zhen,et al."A protein engineering of Bacillus thuringiensis delta-endotoxin by conjugating with 4 ''-O-succinoyl abamectin".http://dx.doi.org/10.1016/j.ijbiomac.2013.08.044 (2013).