Simultaneous Detection, Genotyping, and Quantification of Human Papillomaviruses by Multicolor Real-Time PCR and Melting Curve Analysis

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	Simultaneous Detection, Genotyping, and Quantification of Human Papillomaviruses by Multicolor Real-Time PCR and Melting Curve Analysis
	Liao, Yiqun ; Zhou, Yulin ; Guo, Qiwei ; Xie, Xiaoting ; Luo, Ena ; Li, Jian ; Li, Qingge ; Li QG(李庆阁)
刊名	http://dx.doi.org/10.1128/JCM.02115-12
	2013
关键词	TOF MASS-SPECTROMETRY RAPID-CYCLE PCR FACTOR-V-LEIDEN CERVICAL-CANCER VIRAL LOAD HPV TEST RISK CLASSIFICATION IDENTIFICATION HYBRIDIZATION
英文摘要	Key Project of Science and Technology Program of Fujian [2009D024]; Long-term infection with high-risk human papillomavirus (HPV) is the leading cause of cervical cancer, while infection with low-risk HPV is the major reason for condylomata acuminata. An accurate, rapid, and convenient assay that is able to simultaneously detect, genotype, and quantify HPV would be of great clinical value yet remains to be achieved. We developed a three-color real-time PCR assay that is able to analyze 30 predominant HPV types in three reactions. The amplification curves indicated the presence of HPV, melting curve analysis identified the HPV genotype, and the quantification cycle value determined the quantity. We applied this assay to 647 cervical swab samples, and the results were compared with those obtained with a commercial genotyping system. The proposed assay had a limit of detection of 5 to 50 copies per reaction and a dynamic range of 5 x 10(1) to 5 x 10(6) copies per reaction. A comparison study showed that the overall sample concordance with the comparison method was 91.6% and the type agreement was greater than 98.7%. The quantification study demonstrated that the loads of HPV type 16 in 30 samples with cervical intraepithelial neoplasia grade III (CIN III) lesions were significantly higher than those in samples with CIN I lesions or CIN II lesions, and the results were concordant with those of the comparison method. The increased information content, high throughput, and low cost would facilitate the use of this real-time PCR-based assay in a variety of clinical settings.
语种	英语
出版者	AMER SOC MICROBIOLOGY
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90617]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Liao, Yiqun,Zhou, Yulin,Guo, Qiwei,et al. Simultaneous Detection, Genotyping, and Quantification of Human Papillomaviruses by Multicolor Real-Time PCR and Melting Curve Analysis[J]. http://dx.doi.org/10.1128/JCM.02115-12,2013.
APA	Liao, Yiqun.,Zhou, Yulin.,Guo, Qiwei.,Xie, Xiaoting.,Luo, Ena.,...&李庆阁.(2013).Simultaneous Detection, Genotyping, and Quantification of Human Papillomaviruses by Multicolor Real-Time PCR and Melting Curve Analysis.http://dx.doi.org/10.1128/JCM.02115-12.
MLA	Liao, Yiqun,et al."Simultaneous Detection, Genotyping, and Quantification of Human Papillomaviruses by Multicolor Real-Time PCR and Melting Curve Analysis".http://dx.doi.org/10.1128/JCM.02115-12 (2013).