A novel JNK from Litopenaeus vannamei involved in white spot syndrome virus infection

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	A novel JNK from Litopenaeus vannamei involved in white spot syndrome virus infection
	Shi, Hong ; Yan, Xinfu ; Ruan, Lingwei ; Xu, Xun ; Xu X(徐洵)
刊名	http://dx.doi.org/10.1016/j.dci.2012.03.002
	2012-07
关键词	N-TERMINAL KINASE SIGNALING PATHWAYS MAP KINASES IMMUNE-RESPONSE PROTEIN-KINASES KAPPA-B ACTIVATION JUN REPLICATION STRESS
英文摘要	National Basic Research Program of China (973 Program) [2012CB114403]; National High Technology Research and Development Program of China (863 Program) [2012AA092205]; China Agriculture Research System-47; National Natural Science Foundation of China [30830084]; Scientific Research Foundation of Third Institute of Oceanography, SOA [2011018]; The c-Jun N-terminal kinase (JNK), a member of MAP kinases, is a serine/threonine-specific protein kinase which responds to extracellular stimuli and regulate various cellular activities. It is well documented in innate immune responses and reported to be involved in various viral infections of mammals. In present study, we cloned INK homolog in a crustacean, Litopenaeus vannamei (designated as LvJNK) and studied its role in white spot syndrome virus (WSSV) infection. Sequence analysis displayed that LvJNK shared high similarity with other members of the JNK subfamily, including the conserved TPY motif and serine/threonine protein kinase (S_TKc) domain. Western blot analysis showed that the activation of LvJNK took place in WSSV infection. Lvjnk silencing mediated by specific dsRNA in shrimps could significantly inhibit the proliferation of the virus. Moreover, inhibition of shrimp JNK signaling pathway by specific inhibitor resulted in the reduction of WSSV replication and the delay of WSSV gene transcription. These results indicate for the first time that shrimp JNK is activated in response to WSSV infection and WSSV could benefit from JNK activation. It may facilitate our understanding of the molecular mechanism of virus infection and provided a potential target for preventing the WSSV infection. (c) 2012 Elsevier Ltd. All rights reserved.
语种	英语
出版者	DEV COMP IMMUNOL
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90457]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Shi, Hong,Yan, Xinfu,Ruan, Lingwei,et al. A novel JNK from Litopenaeus vannamei involved in white spot syndrome virus infection[J]. http://dx.doi.org/10.1016/j.dci.2012.03.002,2012.
APA	Shi, Hong,Yan, Xinfu,Ruan, Lingwei,Xu, Xun,&徐洵.(2012).A novel JNK from Litopenaeus vannamei involved in white spot syndrome virus infection.http://dx.doi.org/10.1016/j.dci.2012.03.002.
MLA	Shi, Hong,et al."A novel JNK from Litopenaeus vannamei involved in white spot syndrome virus infection".http://dx.doi.org/10.1016/j.dci.2012.03.002 (2012).