Inhibitory effects of hinokitiol on tyrosinase activity and melanin biosynthesis and its antimicrobial activities

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	Inhibitory effects of hinokitiol on tyrosinase activity and melanin biosynthesis and its antimicrobial activities
	Zhu, Y. J. ; Qiu, L. ; Zhou, J. J. ; Guo, H. Y. ; Hu, Y. H. ; Li, Z. C. ; Wang, Q. ; Chen, Q. X. ; Liu, B. ; Chen QX(陈清西)
刊名	http://dx.doi.org/10.3109/14756360903476398
	2010-12
关键词	MUSHROOM TYROSINASE HUMAN MELANOCYTES MECHANISM PROTEINS
英文摘要	National High Technology Research and Development Program ("863" Program) of China [2006AA10A211]; Natural Science Foundation of China [30570408, 20832005]; Science and Technology Foundation of Fujian Province [2007N0051]; Program for New Century Excellen; The inhibitory effects of hinokitiol, a constituent of the woody oils isolated from Cupressaceae heartwood, on mushroom tyrosinase and melanin formation in B16 melanoma cells as well as its antimicrobial activity were investigated. Our results showed that hinokitiol could strongly inhibit both monophenolase activity and diphenolase activity of the enzyme and the inhibition was reversible. The IC(50) values were estimated as 9.67 mu M for monophenolase activity and 0.21 mu M for diphenolase activity. The lag time of the monophenolase activity was not obviously lengthened by the compound. Kinetic analyses showed that the inhibition mechanism of hinokitiol was a mixed-type inhibition of the diphenolase activity. Hinokitiol effectively inhibited both cellular tyrosinase activity and melanin biosynthesis in B16 melanoma cells with significant cytotoxicity. Furthermore, it was found that hinokitiol could inhibit the proliferation of Salmonella enteritidis, Escherichia coli, Bacillus subtilis, Staphyloccocus aureus, Klebsiella pneumoniae, and Ralstonia solanacearum to different extents. This research may widen the use of hinokitiol in the fields of food preservation, depigmentation, and insecticide use.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65668]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Zhu, Y. J.,Qiu, L.,Zhou, J. J.,et al. Inhibitory effects of hinokitiol on tyrosinase activity and melanin biosynthesis and its antimicrobial activities[J]. http://dx.doi.org/10.3109/14756360903476398,2010.
APA	Zhu, Y. J..,Qiu, L..,Zhou, J. J..,Guo, H. Y..,Hu, Y. H..,...&陈清西.(2010).Inhibitory effects of hinokitiol on tyrosinase activity and melanin biosynthesis and its antimicrobial activities.http://dx.doi.org/10.3109/14756360903476398.
MLA	Zhu, Y. J.,et al."Inhibitory effects of hinokitiol on tyrosinase activity and melanin biosynthesis and its antimicrobial activities".http://dx.doi.org/10.3109/14756360903476398 (2010).