Apoptosis of Human Cholangiocarcinoma Cell Lines induced by beta-Escin through Mitochondrial Caspase-dependent Pathway

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	Apoptosis of Human Cholangiocarcinoma Cell Lines induced by beta-Escin through Mitochondrial Caspase-dependent Pathway
	Shen, Dong-Yan ; Kang, Jin-He ; Song, Wei ; Zhang, Wen-Qing ; Li, Wen-Gang ; Zhao, Yan ; Chen, Qing-Xi ; Chen QX(陈清西)
刊名	http://dx.doi.org/10.1002/ptr.3435
	2011-10
关键词	EPIDEMIOLOGY INFLAMMATION THERAPY DRUGS CYCLE RISK DNA
英文摘要	National Natural Science Foundation of China [81072014]; National Foundation for fostering talents of basic science [J1030626]; The study aimed to evaluate the effects of beta-escin on human cholangiocarcinoma cell lines (QBC939, Sk-ChA-1 and MZ-ChA-1) and to explore its mechanisms. Cell growth, cell cycle and apoptosis were investigated, respectively, by MTT assay, single PI and FITC/PI double-staining flow cytometry, and fluorescence microscopy. The protein expression was determined by western blotting. The study revealed that beta-escin inhibited cholangiocarcinoma cell growth in a dose- and time-dependent manner, and the cell cycle of QBC939 and Sk-ChA-1 cells was arrested in the G2/M phase, and MZ-ChA-1 cells in G1 phase. Apoptosis of the three cholangiocarcinoma cell lines induced by beta-escin was associated with the collapse of the mitochondrial membrane potential and the activation of caspase-3. The apoptotic effect of beta-escin was suppressed by pancaspase inhibitor z-VAD-fmk. Molecular dissection revealed that the antiapoptotic protein bcl-2 was down-regulated after cholangiocarcinoma cell lines were treated with beta-escin, while the protein levels of bax and p53 were unchanged. Apoptosis was accompanied by an increase in reactive oxygen species (ROS). These results suggest that beta-escin induces apoptosis of cholangiocarcinoma cells through an intrinsic mitochondrial caspase-dependent pathway, and the increase in the bax/bcl-2 ratio and ROS may play important roles in beta-escin-induced apoptosis of cholangiocarcinoma cells. Copyright (C) 2011 John Wiley & Sons, Ltd.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65658]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Shen, Dong-Yan,Kang, Jin-He,Song, Wei,et al. Apoptosis of Human Cholangiocarcinoma Cell Lines induced by beta-Escin through Mitochondrial Caspase-dependent Pathway[J]. http://dx.doi.org/10.1002/ptr.3435,2011.
APA	Shen, Dong-Yan.,Kang, Jin-He.,Song, Wei.,Zhang, Wen-Qing.,Li, Wen-Gang.,...&陈清西.(2011).Apoptosis of Human Cholangiocarcinoma Cell Lines induced by beta-Escin through Mitochondrial Caspase-dependent Pathway.http://dx.doi.org/10.1002/ptr.3435.
MLA	Shen, Dong-Yan,et al."Apoptosis of Human Cholangiocarcinoma Cell Lines induced by beta-Escin through Mitochondrial Caspase-dependent Pathway".http://dx.doi.org/10.1002/ptr.3435 (2011).