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	一株高效产油脂真菌的选育及其油脂成分分析; Screening of High Lipid-producing Fungus and Analysis of Composition of the Lipid
	马占军 ; 蔡冠竟 ; 杨旭俊 ; 郑天凌
	2012-11
关键词	海洋真菌 诱变 油脂 脂肪酸 组成分析 marine fungus mutagenesis lipid fatty acid composition analysis
英文摘要	通信作者:wshwzh@xmu.edu.cn; [中文文摘]以早期从海洋环境中分离得到的38株海洋真菌为材料,采用苏丹黑染色法初筛,摇瓶法复筛,得到1株油脂产率相对较高(27.96%)的菌株MD2,经鉴定,该菌株为粘红酵母(Rhodotorula glutinis).再以MD2为出发菌株,经紫外诱变、亚硝基胍诱变和紫外-亚硝基胍复合诱变,最终得到突变菌株MD2-1;采用酸热法提取粘红酵母MD2-1的菌体油脂,测定了其产脂能力,并采用气相色谱-质谱联用法(GC-MS)测定其脂肪酸组成.结果表明:MD2-1的产脂能力较强,油脂质量达菌体干质量的45.32%,较MD2提高17.36%;MD2-1菌体油脂中检测出9种脂肪酸,其脂肪酸组成与植物油近似,其中10-十八(碳)烯酸占脂肪酸总量的71.53%,为10-十八(碳)烯酸工业化生产提供菌种资源,为其规模化生产奠定基础.[英文文摘]Thirty-eight marine fungi strains were isolated from marine environment and subjected to preliminary screening with Sudan Black dye and further screening by shake-flask culture.As a result,strain MD2 was found to have the highest lipid content of 27.96% and identified as Rhodotorula glutinis.The strain was mutagenized respectively with UV light,nitrosoguanidine(NTG) and their combination,obtaining the mutant MD2-1.The lipid was extracted from the strain Rhodotorula glutinis MD2-1 by acid-heat extracting methods,and its fatty acids was analyzed by GC-MS.The results showed that R.glutinis MD2-lexhbited strong ability to produce lipid,and the total amount of lipid represented an average of 45.32% of the dry strain weight..The fatty acid composition of the lipid from R.glutinis MD2-1 was similar to vegetable oil and fat,and the 10-octadecenoic acid accounted for 71.53% of the total fatty acids,so this research made a major significance:provided seed resources for industrial production of 10-octadecenoic acid and laid the foundation for its large-scale production.; 国家自然科学基金重点项目(40930847)、面上项目(31070442); 福建省自然科学基金项目(2012J01150)
语种	中文
出版者	《厦门大学学报(自然科学版)》编辑部
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/17669]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	马占军,蔡冠竟,杨旭俊,等. 一株高效产油脂真菌的选育及其油脂成分分析, Screening of High Lipid-producing Fungus and Analysis of Composition of the Lipid[J],2012.
APA	马占军,蔡冠竟,杨旭俊,&郑天凌.(2012).一株高效产油脂真菌的选育及其油脂成分分析..
MLA	马占军,et al."一株高效产油脂真菌的选育及其油脂成分分析".(2012).

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