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	FISH & SHELLFISH IMMUNOLOGY
	Zhuang, Zhen-Hong(Sun Yat Sen Univ) ; Zhao, Xian-Liang ; Li, Hui(Sun Yat Sen Univ) ; Wang, San-Ying ; Wang SY(王三英) ; Peng, Xuan-Xian
	2011-03-23
关键词	CaVP Amphioxus Immune response
英文摘要	The importance of calcium-binding proteins in immune response of vertebrates is determined, but whether they have the role in invertebrates is largely unknown. In the present study, phylogenetic analysis indicated that calcium vector protein (CaVP), a protein unique to amphioxus, shared 68% similarity in amino acid sequence with human and mouse calmodulin (CaM). CaVP cDNA was cloned into a bacterial vector pET-32a, and its His-tagged fusion protein was produced in Eschherichia coli cells (BL21). The recombinant CaVP was purified by Ni-NTA column and SDS-PAGE, and then utilized for antibody preparing. The prepared antibodies could recognize amphioxus CaVP with high specificity. Further analysis by Western blotting showed that CaVP was detected in muscle and humoral fluid of normal animals and appeared in gut of bacterial immunized or challenged amphioxus. Interestingly, gut CaVP was significantly higher in a healthy sub-group than a wounded sub-group post bacterial challenge. This response was detected strongly in immunization and challenge by the same Gram-negative bacterium Vibro parahaemolyticus and weakly in immunization by V parahaemolyticus and then challenge by Gram-negative Aeromonas hydrophila, whereas no any feedback was found in immunization by V. parahaemolyticus and challenge by Gram-positive Staphylococcus aureus. These findings indicate the importance of gut CaVP in response to bacterial challenge. (C) 2011 Elsevier Ltd. All rights reserved.; NSFC[40876076, 30972279]; [2008AA092603]
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1016/j.fsi.2011.05.004]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Zhuang, Zhen-Hong,Zhao, Xian-Liang,Li, Hui,et al. FISH & SHELLFISH IMMUNOLOGY[J],2011.
APA	Zhuang, Zhen-Hong,Zhao, Xian-Liang,Li, Hui,Wang, San-Ying,王三英,&Peng, Xuan-Xian.(2011).FISH & SHELLFISH IMMUNOLOGY..
MLA	Zhuang, Zhen-Hong,et al."FISH & SHELLFISH IMMUNOLOGY".(2011).

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