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	Proteomic analysis of methyl parathion-responsive proteins in zebrafish (Danio rerio) brain
	Huang, Qing-Yu ; Huang, Lin ; Huang, He-Qing ; Huang HQ(黄河清)
	2010-09-03
关键词	Methyl parathion Zebrafish Brain Proteomics Alteration of protein profiles Biomarkers
英文摘要	Methyl parathion (MP), an organophosphorus pesticide used worldwide, has been associated with a wide spectrum of toxic effects on organisms in the environment. This study set out to analyze the alteration of protein profiles in MP-exposed zebrafish (Danio rerio) brain and find the proteins responsive to MP toxicity. Zebrafish were subjected to 1, 3 and 5 mg/L MP and the proteomic changes in their brains were revealed using two-dimensional gel electrophoresis. Six protein spots were observed to be significantly changed by MP exposure. Among these, 4 spots were down-regulated, while 2 spots were up-regulated. These altered spots were excised from the gels and identified by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry and database searching. The results indicate that these proteins were involved in binding, catalysis, regulation of energy metabolism and cell structure. These data may provide novel biomarkers for the evaluation of MP contamination and useful insights for understanding the mechanisms of MP toxicity. (C) 2010 Elsevier Inc. All rights reserved.; State Natural Science Fund[40776060]; 973 Projects, China[2010CB12640]
语种	英语
出版者	ELSEVIER SCIENCE INC
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1016/j.cbpc.2010.08.009]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Huang, Qing-Yu,Huang, Lin,Huang, He-Qing,et al. Proteomic analysis of methyl parathion-responsive proteins in zebrafish (Danio rerio) brain[J],2010.
APA	Huang, Qing-Yu,Huang, Lin,Huang, He-Qing,&黄河清.(2010).Proteomic analysis of methyl parathion-responsive proteins in zebrafish (Danio rerio) brain..
MLA	Huang, Qing-Yu,et al."Proteomic analysis of methyl parathion-responsive proteins in zebrafish (Danio rerio) brain".(2010).

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