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	Anisakis pegreffii: A quantitative fluorescence PCR assay for detection in situ
	Fang, Wenzhen ; Fang WZ(方文珍) ; Liu, Fan ; Lin, Junhua ; Xu, Shisan ; Luo, Damin ; Luo DM(罗大民)
	2010-11
关键词	Anisakis pegreffii Food-borne parasitic zoonosis In situ Nematode Second internal transcribed spacer (ITS-2) TaqMan real-time PCR
英文摘要	To facilitate improved diagnosis and detection of the third stage larva (L3) of Anisakis pegreffii from the Minnan-Taiwan bank fishing ground in Taiwan Strait, a real-time PCR method for the detection in situ and differentiation was developed to amplify a region of the second internal transcribed spacer (ITS-2) of this parasite. The real-time PCR assay was capable of detecting 1/3 of a single 13 in 30 mg of marine fish tissue, and also exhibited a high level of specificity for A. pegreffii, no fluorescence signals were observed in other five major larval anisakid species found in commercial marine fishes caught in this fishing ground. (C) 2010 Elsevier Inc. All rights reserved.; Science and Technology Planning Project of Fujian Province[2008N2005]; Natural Science Foundation of Fujian Province[2010J01229]; National Foundation for fostering talents of basic science[J1030626]
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1016/j.exppara.2010.11.008]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Fang, Wenzhen,Fang WZ,Liu, Fan,et al. Anisakis pegreffii: A quantitative fluorescence PCR assay for detection in situ[J],2010.
APA	Fang, Wenzhen.,方文珍.,Liu, Fan.,Lin, Junhua.,Xu, Shisan.,...&罗大民.(2010).Anisakis pegreffii: A quantitative fluorescence PCR assay for detection in situ..
MLA	Fang, Wenzhen,et al."Anisakis pegreffii: A quantitative fluorescence PCR assay for detection in situ".(2010).

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