Applications of homemade kit in mutation detection of genes

	Applications of homemade kit in mutation detection of genes
	Zhao, CX ; Xu, GW ; Shi, XZ ; Ma, JM ; Zhang, Y ; Lu, S ; Yang, Q
刊名	science in china series b-chemistry
	2004-04-01
卷号	47 期号:2 页码:166-172
关键词	single nucleotide polymorphism (SNP) single-strand conformation pollymorphism (SSCP) heteroduplex analysis (HA) SNaPshot linear polyacrylamide (LPA) polydimethylacrylamide (PDMA)
产权排序	1;1
英文摘要	several methods of mutation detection, such as single-strand conformation polymorphism (sscp), tandem sscp/heteroduplex analysis and snapshot analysis were developed using homemade kit on abi 310 genetic analyzer, and were successfully applied to mutation detection of 31 colorectal tumor samples. the sieving capability of homemade kit and commercial kit were compared, results demonstrate that homemade kit has higher resolution and shorter analysis time. in clinical tumor samples, 26% k-ras (exon 1) and 24% p53 (exons 7-8) were found to have mutations, and all mutations were single point variations. a majority of mutations occurred in one gene, only 1 tumor contained alterations in the two genes, which indicates that development of colorectal cancer lies on alternate pathways, and may correlate with different gene mutations.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, multidisciplinary
研究领域[WOS]	chemistry
关键词[WOS]	strand conformation polymorphism ; single nucleotide polymorphisms ; capillary-electrophoresis ; gel-electrophoresis ; dna separation ; resolution ; polymers
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000221119100012
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/81587]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China 2.Dalian Med Univ, Affiliated Hosp 2, Cell Lab, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Zhao, CX,Xu, GW,Shi, XZ,et al. Applications of homemade kit in mutation detection of genes[J]. science in china series b-chemistry,2004,47(2):166-172.
APA	Zhao, CX.,Xu, GW.,Shi, XZ.,Ma, JM.,Zhang, Y.,...&Yang, Q.(2004).Applications of homemade kit in mutation detection of genes.science in china series b-chemistry,47(2),166-172.
MLA	Zhao, CX,et al."Applications of homemade kit in mutation detection of genes".science in china series b-chemistry 47.2(2004):166-172.