| 微生物清防蜡技术研究及应用; Research and Application of Microbial Paraffin-removal Technology | |
| 刘江红 ; 贾云鹏 ; 徐瑞丹 ; 陈逸桐 ; 王鉴 | |
| 2013-05-25 | |
| 关键词 | 微生物 芽孢杆菌属 蜡 降解 原油 microorganisms Bacillus sp paraffins degradation crude oil |
| 英文摘要 | 利用从大庆含蜡原油中分离、纯化得到的微生物清防蜡菌种和高产表活剂菌种,经鉴定清防蜡菌种和高产表活剂菌种均为芽孢杆菌属.以菌种对固体石蜡的降解率为指标,按照不同的比例将清防蜡菌种和高产表活剂菌种混合接种.当清防蜡菌种与高产表活剂菌种的复配比例是5∶3时,培养7d后,清蜡率达到59%,防蜡率达到57.4%,原油粘度降粘率为44.7%,原油凝固点降低了3.4℃,培养液表面张力降低46.5%.采用微生物清防蜡技术对大庆外围榆树林油田的3口井进行现场试验,井12-36日产油增长41.2%,洗井周期由40d延长至149d,减少洗井次数4次;井13-39日产油增长33.3%,洗井周期由45d延长至158d,减少洗井次数5次;井14-43日产油增长37.5%,洗井周期由30d延长至122d,减少洗井次数5次.; Microorganisms were obtained by means of separation and purification experiments from waxy oil production wells in Daqing Oilfield.The paraffin-degrading strain and the biosurfactant-producing strain were identified as Bacillus sp..As the indicator of the paraffin degradation rate,the paraffin-degrading strain and biosurfactant-producing strain were added in different proportions.When the optimum proportion was 5∶3,the paraffin degradation rate could reach 59%,the paraffin prevention rate could reach 57.4%,the reduction rate of oil viscosity was 44.7%,the oil freezing point reduced by 3.4℃,and the reduction rate of culture surface tension was 46.5%after 7days.Microbial paraffin-removal technology was used for paraffin removal in Wells 12-36,13-39and 14-43in Yushulin Zone Daqing Oilfield.The results indicated that the daily crude oil increased by 41.2%,hot washing cycles prolonged from 40dto 149d and washing numbers reduced by 4times in Well 12-36,the daily crude oil increased by 33.3%,hot washing cycles prolonged from 45dto 158dand hot washing numbers reduced by 5times in Well 13-39,the daily crude oil increased by 37.5%,hot washing cycles prolonged from 30dto 122dand hot washing numbers reduced by 5times in Well 14-43.; 黑龙江省教育厅科学技术研究项目(12531064) |
| 语种 | zh_CN |
| 内容类型 | 期刊论文 |
| 源URL | [http://dspace.xmu.edu.cn/handle/2288/106884]  |
| 专题 | 化学化工－已发表论文 |
| 推荐引用方式 GB/T 7714 | 刘江红,贾云鹏,徐瑞丹,等. 微生物清防蜡技术研究及应用, Research and Application of Microbial Paraffin-removal Technology[J],2013. |
| APA | 刘江红,贾云鹏,徐瑞丹,陈逸桐,&王鉴.(2013).微生物清防蜡技术研究及应用.. |
| MLA | 刘江红,et al."微生物清防蜡技术研究及应用".(2013). |
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