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	原花青素抑制脂多糖诱导的RAW264.7细胞PGE_2生成的机制; The mechanisms of proanthocyanidins Inhibits PGE_2 synthesis in lipopolysaccharide-Induced RAW264.7 cells
	陈美珺 ; 梁统 ; 周克元
	2009
关键词	原花青素 环氧化酶-2 前列腺素E2 核转录因子-κB 电泳迁移率变动分析 Proanthocyanidins Cyclooxygenase-2 Prostaglandin E2 Nuclear factor of kappa B(NF-κB) Electrophretic mobility shift assay(EMSA)
英文摘要	目的:观察原花青素对脂多糖诱导rAW264.7细胞PgE2生成的影响及其作用机制。方法:放射免疫法(rIA)检测原花青素对脂多糖诱导的rAW264.7细胞PgE2生成的影响;lPS诱导rAW264.7细胞9H,再加不同浓度原花青素作用30MIn,放射免疫(rIA)法检测原花青素对COX-2酶活性的影响;半定量逆转录-聚合酶联反应(rT-PCr)法检测原花青素对COX-2 MrnA表达的影响;提取核蛋白,蛋白免疫印迹(WESTErn blOT)法检测原花青素对nf-κb/P65蛋白表达的影响;电泳迁移率变动分析(EMSA)法检测原花青素对nf-κb与dnA结合活性的影响。结果:0.8,4和20Mg·l-1原花青素抑制lPS诱导rAW264.7细胞PgE2生成;0.8,4和20Mg·l-1原花青素不影响lPS诱导rAW264.7细胞COX-2酶活性;0.8,4和20Mg·l-1原花青素下调lPS诱导rAW264.7细胞COX-2 MrnA表达;4,20Mg·l-1原花青素下调lPS诱导rAW264.7细胞nf-κb/P65蛋白表达;0.8,4和20Mg·l-1的原花青素可明显降低lPS诱导下rAW264.7细胞的nf-κb活化。结论:原花青素显著抑制lPS诱导rAW264.7细胞PgE2生成的作用与原花青素抑制COX-2 MrnA表达有关,此作用可能是通过抑制nf-κb/P65蛋白表达和抑制nf-κb的dnA结合活性来实现。; Objective:To study the effect of proanthocyanidins on PGE2 synthesis in lipopolysaccharide-induced RAW264.7 cells and its mechanisms.Methods: The effect of proanthocyanidins on PGE2 synthesis in lipopolysaccharide-induced RAW264.7 cells was measured by radioimmunoassay(RIA);After being pretreated with different concentrations of proanthocyanidins for 30 min,and then LPS 1 mg·L-1 for 9 h,the effect of proanthocyanidins on the activity of COx-2 enzyme in RAW264.7 cells was analysed by RIA;the expressions of COx-2 mRNA by RT-PCR;the nuclear protein was isolated and the expressions of NF-κB protein by Western blot;and the DNA-binding activity of NF-κB was measured by electrophoretic mobility shift assay(EMSA).Results: PGE2 synthesis was inhibited by proanthocyanidins 0.8 mg·L-1、4 mg·L-1 and 20 mg·L-1 ;the activity of COx-2 enzyme was not inhibited by proanthocyanidins 0.8 mg·L-1、4 mg·L-1 and 20 mg·L-1 (P>0.05,vs LPS group);the expression of COx-2 mRNA was inhibited by proanthocyanidins 0.8 mg·L-1、4 mg·L-1 and 20 mg·L-1;the expression of NF-κB/p65 protein was inhibited by proanthocyanidins 4 mg·L-1 and 20 mg·L-1.The DNA-binding activity of NF-κB was reduced by proanthocyanidins 0.8 mg·L-1、4 mg·L-1 and 20 mg·L-1.Conclusion: The mechanisms of proanthocyanidins inhibited PGE2 synthesis in lipopolysaccharide-induced RAW264.7 cells is related to the inhibition of the expression of COx-2 mRNA ,which is inhibited possibly by suppressing expression of NF-κB/p65 protein and DNA-binding activity of NF-κB.; 广东省教育厅自然科学基金(Z03045)----
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/106294]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	陈美珺,梁统,周克元. 原花青素抑制脂多糖诱导的RAW264.7细胞PGE_2生成的机制, The mechanisms of proanthocyanidins Inhibits PGE_2 synthesis in lipopolysaccharide-Induced RAW264.7 cells[J],2009.
APA	陈美珺,梁统,&周克元.(2009).原花青素抑制脂多糖诱导的RAW264.7细胞PGE_2生成的机制..
MLA	陈美珺,et al."原花青素抑制脂多糖诱导的RAW264.7细胞PGE_2生成的机制".(2009).

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