Label-free fluorescence strategy for sensitive detection of adenosine triphosphate using a loop DNA probe with low background noise

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	Label-free fluorescence strategy for sensitive detection of adenosine triphosphate using a loop DNA probe with low background noise
	Lin, Chunshui ; Cai, Zhixiong ; Wang, Yiru ; Zhu, Zhi ; Yang, Chaoyong James ; Chen, ; Wang YR(王翊如) ; Zhu Z(朱志) ; Yang CY(杨朝勇) ; Chen X(陈曦)
刊名	http://dx.doi.org/10.1021/ac501730u
	2014
关键词	Adenosinetriphosphate DNA Phosphates Probes Signal detection
英文摘要	A simple, rapid, label-free, and ultrasensitive fluorescence strategy for adenosine triphosphate (ATP) detection was developed using a loop DNA probe with low background noise. In this strategy, a loop DNA probe, which is the substrate for both ligation and digestion enzyme reaction, was designed. SYBR green I (SG I), a double-stranded specific dye, was applied for the readout fluorescence signal. Exonuclease I (Exo I) and exonuclease III (Exo III), sequence-independent nucleases, were selected to digest the loop DNA probe in order to minimize the background fluorescence signal. As a result, in the absence of ATP, the loop DNA was completely digested by Exo I and Exo III, leading to low background fluorescence owing to the weak electrostatic interaction between SG I and mononucleotides. On the other hand, ATP induced the ligation of the nicking site, and the sealed loop DNA resisted the digestion of Exo I and ExoIII, resulting in a remarkable increase of fluorescence response. Upon background noise reduction, the sensitivity of the ATP determination was improved significantly, and the detection limitation was found to be 1.2 pM, which is much lower than that in almost all the previously reported methods. This strategy has promise for wide application in the determination of ATP. ? 2014 American Chemical Society.
语种	英语
出版者	AMER CHEMICAL SOC
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/89807]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Lin, Chunshui,Cai, Zhixiong,Wang, Yiru,et al. Label-free fluorescence strategy for sensitive detection of adenosine triphosphate using a loop DNA probe with low background noise[J]. http://dx.doi.org/10.1021/ac501730u,2014.
APA	Lin, Chunshui.,Cai, Zhixiong.,Wang, Yiru.,Zhu, Zhi.,Yang, Chaoyong James.,...&陈曦.(2014).Label-free fluorescence strategy for sensitive detection of adenosine triphosphate using a loop DNA probe with low background noise.http://dx.doi.org/10.1021/ac501730u.
MLA	Lin, Chunshui,et al."Label-free fluorescence strategy for sensitive detection of adenosine triphosphate using a loop DNA probe with low background noise".http://dx.doi.org/10.1021/ac501730u (2014).