Target-responsive DNAzyme cross-linked hydrogel for visual quantitative detection of lead

CORC > 厦门大学 > 化学化工－已发表论文

	Target-responsive DNAzyme cross-linked hydrogel for visual quantitative detection of lead
	Huang, Yishun ; Ma, Yanli ; Chen, Yahong ; Wu, Xuemeng ; Fang, Luting ; Zhu, Zhi ; Yang, Chaoyong James ; Zhu Z(朱志) ; Yang CY(杨朝勇)
刊名	http://dx.doi.org/10.1021/ac503540q
	2014
关键词	Chemical detection Colorimetric analysis Gold Health risks Lead Nanoparticles Pollution detection
英文摘要	Because of the severe health risks associated with lead pollution, rapid, sensitive, and portable detection of low levels of Pb2+ in biological and environmental samples is of great importance. In this work, a Pb2+-responsive hydrogel was prepared using a DNAzyme and its substrate as cross-linker for rapid, sensitive, portable, and quantitative detection of Pb2+. Gold nanoparticles (AuNPs) were first encapsulated in the hydrogel as an indicator for colorimetric analysis. In the absence of lead, the DNAzyme is inactive, and the substrate cross-linker maintains the hydrogel in the gel form. In contrast, the presence of lead activates the DNAzyme to cleave the substrate, decreasing the cross-linking density of the hydrogel and resulting in dissolution of the hydrogel and release of AuNPs for visual detection. As low as 10 nM Pb2+ can be detected by the naked eye. Furthermore, to realize quantitative visual detection, a volumetric bar-chart chip (V-chip) was used for quantitative readout of the hydrogel system by replacing AuNPs with gold-platinum core-shell nanoparticles (Au@PtNPs). The Au@PtNPs released from the hydrogel upon target activation can efficiently catalyze the decomposition of H2O2 to generate a large volume of O2. The gas pressure moves an ink bar in the V-chip for portable visual quantitative detection of lead with a detection limit less than 5 nM. The device was able to detect lead in digested blood with excellent accuracy. The method developed can be used for portable lead quantitation in many applications. Furthermore, the method can be further extended to portable visual quantitative detection of a variety of targets by replacing the lead-responsive DNAzyme with other DNAzymes. ? 2014 American Chemical Society.
语种	英语
出版者	American Chemical Society
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/89741]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Huang, Yishun,Ma, Yanli,Chen, Yahong,et al. Target-responsive DNAzyme cross-linked hydrogel for visual quantitative detection of lead[J]. http://dx.doi.org/10.1021/ac503540q,2014.
APA	Huang, Yishun.,Ma, Yanli.,Chen, Yahong.,Wu, Xuemeng.,Fang, Luting.,...&杨朝勇.(2014).Target-responsive DNAzyme cross-linked hydrogel for visual quantitative detection of lead.http://dx.doi.org/10.1021/ac503540q.
MLA	Huang, Yishun,et al."Target-responsive DNAzyme cross-linked hydrogel for visual quantitative detection of lead".http://dx.doi.org/10.1021/ac503540q (2014).