In Situ Study of the Antibacterial Activity and Mechanism of Action of Silver Nanoparticles by Surface-Enhanced Raman Spectroscopy

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	In Situ Study of the Antibacterial Activity and Mechanism of Action of Silver Nanoparticles by Surface-Enhanced Raman Spectroscopy
	Cui, Li ; Chen, Pengyu ; Chen, Shaode ; Yuan, Zhihua ; Yu, Changping ; Ren, Bin ; Zhang, Kaisong ; Ren B(任斌)
刊名	http://dx.doi.org/10.1021/ac400245j
	2013
关键词	CHEMICAL-COMPOSITION ESCHERICHIA-COLI BACTERIA SCATTERING CELLS SERS ANTIBIOTICS INFORMATION METABOLISM ADSORPTION
英文摘要	National Natural Science Foundation of China [20903076, 21173208]; 973 Program [2013CB933703]; Silver nanoparticles (Ag NPs) are extensively used as an antibacterial additive in commercial products and their release has caused environmental risk. However, conventional methods for the toxicity detection of Ag NPs are very time consuming and the mechanisms of action are not clear. We developed a new, in situ, rapid, and sensitive fingerprinting approach, using surface-enhanced Raman spectroscopy (SERS), to study the antibacterial activity and mechanism of Ag NPs of 80 and 18 nm (Ag80 and Ag18, respectively), by using the strong electromagnetic enhancement generated by Ag NPs. Sensitive spectra changes representing various biomolecules in bacteria were observed with increasing concentrations of Ag NPs. They not only allowed SERS to monitor the antibacterial activity of Ag NPs of different sizes in different water media but also to study the antibacterial mechanism at the molecular level. Ag18 were found to be more toxic than Ag80 in water, but their toxicity declined to a similar level in the PBS medium. The antibacterial mechanism was proposed on the basis of a careful identification of the chemical origins by comparing the SERS spectra with model compounds. The dramatic change in protein, hypoxanthine, adenosine, and guanosine bands suggested that Ag NPs have a significant impact on the protein and metabolic processes of purine. Finally, by adding nontoxic and SERS active Au NPs, SERS was successfully utilized to study the action mode of the NPs unable to produce an observable SERS signal. This work opens a window for the future extensive SERS studies of the antibacterial mechanism of a great variety of non-SERS-active NPs.
语种	英语
出版者	AMER CHEMICAL SOC
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/89014]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Cui, Li,Chen, Pengyu,Chen, Shaode,et al. In Situ Study of the Antibacterial Activity and Mechanism of Action of Silver Nanoparticles by Surface-Enhanced Raman Spectroscopy[J]. http://dx.doi.org/10.1021/ac400245j,2013.
APA	Cui, Li.,Chen, Pengyu.,Chen, Shaode.,Yuan, Zhihua.,Yu, Changping.,...&任斌.(2013).In Situ Study of the Antibacterial Activity and Mechanism of Action of Silver Nanoparticles by Surface-Enhanced Raman Spectroscopy.http://dx.doi.org/10.1021/ac400245j.
MLA	Cui, Li,et al."In Situ Study of the Antibacterial Activity and Mechanism of Action of Silver Nanoparticles by Surface-Enhanced Raman Spectroscopy".http://dx.doi.org/10.1021/ac400245j (2013).