Mammalian cell-adhesion kinetics measured by suspension depletion

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	Mammalian cell-adhesion kinetics measured by suspension depletion
	Huang, Qiaoling ; Cheng, Alice ; Antensteiner, Martin ; Lin, Changjian ; Vogler, Erwin A. ; Lin CJ(林昌健)
刊名	http://dx.doi.org/10.1016/j.biomaterials.2012.09.073
	2013
关键词	ATTACHMENT SURFACES WATER MICROCARRIERS REGENERATION INVITRO SILICA
英文摘要	National Natural Science Foundation of China [21021002]; National Scientific Support Program of China [2012BAI07B09]; Mammalian cell-adhesion kinetics is measured by counting the number of cells lost from suspension due to adhesion to planar or particulate substrata as a function of time rather than by the counting of adherent cells that is widely applied in the literature. A simple statistical model shows that this "suspension-depletion" method is most accurate at low cell counts in the critical early stage of cell adhesion that is diagnostic of forces in close proximity between cell and substratum responsible for cell adhesion. Furthermore, suspension depletion avoids experimental artifacts associated with substratum rinsing and the removal of cells from the substratum using enzymatic and/or mechanical methods. Experimental method is demonstrated for three different cell types (MDCK, epithelioid; MC3T3-E1, pre-osteoblast; MDA-MB-231, human breast tumor) adhering to seven different substrata incrementally sampling the observable water-wettability range in Petri-dish format, as well as MDCK adhesion to particulate carriers in stirred suspension. Suspension depletion is ideal for biocompatibility and fouling studies where quantification of "low-and-slow" cell adhesion is important. In particular, it is shown that a typical method of counting adherent cells does not correctly measure adhesion kinetics to hydrophobic surfaces that are generally resistant to mammalian cell adhesion. (C) 2012 Elsevier Ltd. All rights reserved.
语种	英语
出版者	ELSEVIER SCI LTD
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88879]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Huang, Qiaoling,Cheng, Alice,Antensteiner, Martin,et al. Mammalian cell-adhesion kinetics measured by suspension depletion[J]. http://dx.doi.org/10.1016/j.biomaterials.2012.09.073,2013.
APA	Huang, Qiaoling,Cheng, Alice,Antensteiner, Martin,Lin, Changjian,Vogler, Erwin A.,&林昌健.(2013).Mammalian cell-adhesion kinetics measured by suspension depletion.http://dx.doi.org/10.1016/j.biomaterials.2012.09.073.
MLA	Huang, Qiaoling,et al."Mammalian cell-adhesion kinetics measured by suspension depletion".http://dx.doi.org/10.1016/j.biomaterials.2012.09.073 (2013).