Rapid finding and quantification of the major antioxidant in water extracts of three marine drug organisms from China by online HPLC-DAD/MS-DPPH

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	Rapid finding and quantification of the major antioxidant in water extracts of three marine drug organisms from China by online HPLC-DAD/MS-DPPH
	Zheng, Li ; Chen, Junhui ; Zhao, Hengqiang ; Shi, Qian ; Yang, Baijuan ; Cheng, Hongyan ; Zang, Jiaye ; Wang, Xiaoru ; Wang XR(王小如)
刊名	http://dx.doi.org/10.1080/14786419.2011.565285
	2012
关键词	RADICAL SCAVENGING COMPOUNDS LIQUID-CHROMATOGRAPHY SCREENING METHOD MASS-SPECTROMETRY ACACIA-CONFUSA IDENTIFICATION ABTS PHYTOCHEMICALS SEAHORSE ASSAY
英文摘要	National Natural Scientific Foundation of China [20905017, 41076108]; Key Project of Commonwealth Programs for Marine Science [201005034-3, 200805039]; Primary Scientific Foundation of FIO [2008T32, 2010G25]; Science Foundation for Youth Scholars of State Oceanic Administration [2010140]; Ministry of Science and Technology of China [2007AA092001-10]; Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences; A method based on high-performance liquid chromatography (HPLC) with diode array detector coupled with electrospray ionisation-mass spectrometry and an online detection system for radical scavenging was established and used to rapidly find and quantify antioxidant compounds in the water extracts of Hippocampus japonicus Kaup, Hippocampus kuda Bleeker and Syngnathus acus Linnaeus. The online screening results revealed the presence of one major radical scavenging compound identified as hypoxanthine by comparison of mass data and retention time with the standard. Subsequently, the developed HPLC method was applied to quantify hypoxanthine in different H. japonicus, H. kuda and S. acus samples. The results indicated that the developed HPLC method is simple and reliable for the quantification of hypoxanthine with a detection limit at 0.002 mu gmL(-1), and a high recovery from 96.3% to 102.1%. This method provides a powerful tool for rapid identification and quantification of free radical scavenging compounds in complex marine natural products.
语种	英语
出版者	NAT PROD RES
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88581]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Zheng, Li,Chen, Junhui,Zhao, Hengqiang,et al. Rapid finding and quantification of the major antioxidant in water extracts of three marine drug organisms from China by online HPLC-DAD/MS-DPPH[J]. http://dx.doi.org/10.1080/14786419.2011.565285,2012.
APA	Zheng, Li.,Chen, Junhui.,Zhao, Hengqiang.,Shi, Qian.,Yang, Baijuan.,...&王小如.(2012).Rapid finding and quantification of the major antioxidant in water extracts of three marine drug organisms from China by online HPLC-DAD/MS-DPPH.http://dx.doi.org/10.1080/14786419.2011.565285.
MLA	Zheng, Li,et al."Rapid finding and quantification of the major antioxidant in water extracts of three marine drug organisms from China by online HPLC-DAD/MS-DPPH".http://dx.doi.org/10.1080/14786419.2011.565285 (2012).