Fluoresence immunoassay of alpha-fetoprotein with iron(III) tetrasulfonatophthalocyanine as a mimetic enzyme labeling reagent

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	Fluoresence immunoassay of alpha-fetoprotein with iron(III) tetrasulfonatophthalocyanine as a mimetic enzyme labeling reagent
	Yang, HH ; Zhu, QZ ; Li, DH ; Lin, P ; Ding, MT ; Xu, JG ; Yang HH(杨黄浩) ; Li DH(李东辉) ; Zhu QZ(朱庆枝) ; Lin P(林鹏) ; Xu JG(许金钩)
刊名	http://dx.doi.org/10.1007/s002160100723
	2001-05
关键词	HYDROGEN-PEROXIDE SUBSTRATE THIAMINE POLYMER ACID
英文摘要	A new fluorimetric immunoassay for a-fetoprotein (AFP) has been developed using a novel promising mimetic peroxidase, iron(III) tetrasulfonatophthalocyanine (FeTSPc). as a labeling reagent to catalyze the fluorescence reaction of P-hydroxyphenylacetic acid (P-HPA) and hydrogen peroxide (H2O2) In the competitive immunoassay, anti-AFP antibody was coated on a 96-well plate (polystyrene) and a constant amount of FeTSPc-labeled AFP and a known amount of test solution were added. Non-labeled and FeTSPc-labeled AFP compete for binding to the plate-bound antibody. After the immunoreaction, the immunochemically adsorbed FeTSPc-AFP conjugate moiety was determined by measuring the fluorescence produced in a solution containing P-HPA and H2O2. AFP can be determined in the concentration range of 1-300 ng mL(-1) with a detection limit of 0.5 ng mL(-1).
语种	英语
出版者	FRESEN J ANAL CHEM
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88496]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Yang, HH,Zhu, QZ,Li, DH,et al. Fluoresence immunoassay of alpha-fetoprotein with iron(III) tetrasulfonatophthalocyanine as a mimetic enzyme labeling reagent[J]. http://dx.doi.org/10.1007/s002160100723,2001.
APA	Yang, HH.,Zhu, QZ.,Li, DH.,Lin, P.,Ding, MT.,...&许金钩.(2001).Fluoresence immunoassay of alpha-fetoprotein with iron(III) tetrasulfonatophthalocyanine as a mimetic enzyme labeling reagent.http://dx.doi.org/10.1007/s002160100723.
MLA	Yang, HH,et al."Fluoresence immunoassay of alpha-fetoprotein with iron(III) tetrasulfonatophthalocyanine as a mimetic enzyme labeling reagent".http://dx.doi.org/10.1007/s002160100723 (2001).