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	Determination of nucleic acids at nanogram levels with tetra-substituted amino aluminum phthalocyanine by a resonance light-scattering technique
	Chen, XL ; Li, DH ; Zhu, QZ ; Yang, HH ; Zheng, H ; Xu, JG ; Zheng H(郑洪)
	2001-06
关键词	DOUBLE-STRANDED DNA DYES BINDING
英文摘要	This is the first report of the determination of nucleic acids at nanogram levels based on their enhanced effects on the resonance light-scattering of TAA1Pc at 400 nm, Under optimal conditions, the linear ranges of the calibration curves were 0-250.0 ng/mL for Calf thymus DNA (CT DNA), 0-200, 0 ng/mL for Salmon sperm DNA(SM DNA) and 0-400. 0 ng/mL for Yeast RNA. The detection limits were 1. 4 ng/mL for both CT DNA and SM DNA, 2. 7 ng/mL for Yeast RNA. The relative standard deviation for the determination of 100. 0 ng/mL CT DNA was 2. 1%. The measurement can be made easily on a common spectrofluorimeter, The reaction between TAA1Pc and nucleic acids is completed in 2 min and the scattered light signal is stable for at least I h, The interference from some interesting coexisting substances with the determination of DNA was also examined. The method was applied to the determination of DNA in golden staphylococcus DNA sample, and the results were satisfactory.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/64046]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Chen, XL,Li, DH,Zhu, QZ,et al. Determination of nucleic acids at nanogram levels with tetra-substituted amino aluminum phthalocyanine by a resonance light-scattering technique[J],2001.
APA	Chen, XL.,Li, DH.,Zhu, QZ.,Yang, HH.,Zheng, H.,...&郑洪.(2001).Determination of nucleic acids at nanogram levels with tetra-substituted amino aluminum phthalocyanine by a resonance light-scattering technique..
MLA	Chen, XL,et al."Determination of nucleic acids at nanogram levels with tetra-substituted amino aluminum phthalocyanine by a resonance light-scattering technique".(2001).

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