Integrin-Targeted Trifunctional Probe for Cancer Cells: A 'Seeing and Counting' Approach

CORC > 厦门大学 > 化学化工－已发表论文

	Integrin-Targeted Trifunctional Probe for Cancer Cells: A 'Seeing and Counting' Approach
	Zhang, Zhubao ; Luo, Qiang ; Yan, Xiaowen ; Li, Zhaoxin ; Luo, Yacui ; Yang, Limin ; Zhang, Bo ; Chen, Haifeng ; Wang, Qiuquan ; Wang QQ(王秋泉)
刊名	http://dx.doi.org/10.1021/ac302029w
	2012-11-06
关键词	QUANTUM DOTS BREAST-CANCER ALPHA(V)BETA(3) QUANTIFICATION ALPHA-V-BETA-3 FLUORESCENCE FIBRONECTIN TOMOGRAPHY EXPRESSION LIGAND
英文摘要	National Natural Science Foundation of China [21035006, 21275120]; National Basic Research 973 Program [2009CB421605]; We report the design and synthesis of a trifunctional probe for seeing and counting cancer cells using both fluorescence imaging (FI) and inductively coupled plasma mass spectrometry (ICPMS) for the first time. It consisted of a guiding cyclic RGD peptide unit to catch cancer cells via targeting the alpha(v)beta(3) integrin overexpressed on their surface, a 5-amino-fluorescein moiety for FI using confocal laser scanning microscopy (CLSM) as well as a 2-aminoethyl-monoamide-DOTA group for loading stable europium ion and subsequent ICPMS quantification of the cancer cells without the use of radioactive isotopes. In addition to FI, the LOD (3 sigma) of the alpha(v)beta(3) integrin was down to 69.2-309.4 amol per cell depending on the type of the alpha(v)beta(3)-positive cancer cells when using ICPMS and those of the cancer cell number reached 17-75. This probe developed enables us not only to see but also to count the alpha(v)beta(3)-positive cancer cells ultrasensitively, paving a new way for early diagnosis of cancer.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/62791]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Zhang, Zhubao,Luo, Qiang,Yan, Xiaowen,et al. Integrin-Targeted Trifunctional Probe for Cancer Cells: A 'Seeing and Counting' Approach[J]. http://dx.doi.org/10.1021/ac302029w,2012.
APA	Zhang, Zhubao.,Luo, Qiang.,Yan, Xiaowen.,Li, Zhaoxin.,Luo, Yacui.,...&王秋泉.(2012).Integrin-Targeted Trifunctional Probe for Cancer Cells: A 'Seeing and Counting' Approach.http://dx.doi.org/10.1021/ac302029w.
MLA	Zhang, Zhubao,et al."Integrin-Targeted Trifunctional Probe for Cancer Cells: A 'Seeing and Counting' Approach".http://dx.doi.org/10.1021/ac302029w (2012).