Rapid simultaneous determination of protoporphyrin IX, uroporphyrin III and coproporphyrin III in human whole blood by non-linear variable-angle synchronous fluorescence technique coupled with partial least squares

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	Rapid simultaneous determination of protoporphyrin IX, uroporphyrin III and coproporphyrin III in human whole blood by non-linear variable-angle synchronous fluorescence technique coupled with partial least squares
	Liu,QA ; Shindi,AAF ; Zhu,EY ; Li,YQ ; Huang,W ; Li YQ(李耀群)
刊名	http://dx.doi.org/10.1016/j.talanta.2010.07.034
	2010-09-15
关键词	POLYCYCLIC AROMATIC-HYDROCARBONS TANDEM MASS-SPECTROMETRY FLUOROMETRIC-DETERMINATION MULTIVARIATE CALIBRATION LIQUID-CHROMATOGRAPHY PORPHYRINS URINE ACID SAMPLES MATRIX
英文摘要	National Natural Science Foundation of China [20975084, 20575055]; National Basic Research Program of China (973Program) [2007CB935600]; Science and Technology Program of Fujian Province [2009Y0046]; Fluorescence spectroscopy provides high sensitivity in quantitative analysis. However, due to spectral interference, it is difficult to determine the individual components of fluorescent multi-component mixtures in such complicated and important body matrices as blood, urine and feces without any preseparation. In this study, a simple and rapid approach based on non-linear variable-angle synchronous fluorescence spectrometry coupled with partial least squares analysis (NLVASF/PLS) was developed for the simultaneous determination of protoporphyrin IX (PP), uroporphyrin III (UP) and coproporphyrin III (CP). The detection limits were 0.18, 0.29 and 0.24 nmol L(-1) for protoporphyrin IX (PP), uroporphyrin III (UP) and coproporphyrin III (CP), respectively. The individual components of blood porphyrins were quantified, by this method, simultaneously in one scan with only about 30 s. The recoveries of this method were above 80% in human whole blood samples. This method provided a potential tool for the determination of porphyrins in whole blood and the differential diagnosis of porphyria, especially for rapid routine screening of large number of samples. (C) 2010 Elsevier B.V. All rights reserved.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/62249]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Liu,QA,Shindi,AAF,Zhu,EY,et al. Rapid simultaneous determination of protoporphyrin IX, uroporphyrin III and coproporphyrin III in human whole blood by non-linear variable-angle synchronous fluorescence technique coupled with partial least squares[J]. http://dx.doi.org/10.1016/j.talanta.2010.07.034,2010.
APA	Liu,QA,Shindi,AAF,Zhu,EY,Li,YQ,Huang,W,&李耀群.(2010).Rapid simultaneous determination of protoporphyrin IX, uroporphyrin III and coproporphyrin III in human whole blood by non-linear variable-angle synchronous fluorescence technique coupled with partial least squares.http://dx.doi.org/10.1016/j.talanta.2010.07.034.
MLA	Liu,QA,et al."Rapid simultaneous determination of protoporphyrin IX, uroporphyrin III and coproporphyrin III in human whole blood by non-linear variable-angle synchronous fluorescence technique coupled with partial least squares".http://dx.doi.org/10.1016/j.talanta.2010.07.034 (2010).