PREPARATION OF A MONOLITH WITH COVALENTLY BOUND BOVINE SERUM ALBUMIN FOR CAPILLARY ELECTROCHROMATOGRAPHY

CORC > 厦门大学 > 化学化工－已发表论文

	PREPARATION OF A MONOLITH WITH COVALENTLY BOUND BOVINE SERUM ALBUMIN FOR CAPILLARY ELECTROCHROMATOGRAPHY
	Yao, Chuanyi ; Tang, Shaokun ; Yan, Chao ; Yao, Hong-Mei ; Tade, Moses O. ; Yao CY(姚传义)
刊名	http://dx.doi.org/10.1080/00032719.2012.689793
	2012
关键词	CHIRAL STATIONARY-PHASE SOL-GEL METHOD SILICA MONOLITH ENANTIOMER SEPARATION AFFINITY MONOLITH LIQUID-CHROMATOGRAPHY COLUMNS CEC SELECTORS AVIDIN
英文摘要	National Natural Science Foundation of China [20876128]; Fundamental Research Funds for the Central Universities [2010121049]; Capillary electrochromatography (CEC) is important for applications in enantiomer separation. The problems associated with column fabrication bring a challenge in developing monoliths with ease of preparation, robustness of separation, enhanced mass transfer, and lower pressure drop. In this research, the covalent binding of proteins on to a monolithic matrix was investigated to overcome the drawback of loss and/or denaturing of the biomolecules from physical adsorption and encapsulation method. A chitosan/silica hybrid monolith was prepared and a protein, bovine serum albumin, was covalently immobilized on the column. The prepared monolith was evaluated using the enantioseparation of D, L-tryptophan by CEC. It was found that separation of tryptophan enantiomers with a resolution of 2.44 was achieved by using 20 mmol L-1 phosphate buffer at pH 7.5. A higher chitosan concentration was also proven to be of possible use in the synthesis with the aid of acetic acid as the solvent. The much shorter retention time and increased separation ability demonstrate the advantages of capillary column under investigation.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/61647]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Yao, Chuanyi,Tang, Shaokun,Yan, Chao,et al. PREPARATION OF A MONOLITH WITH COVALENTLY BOUND BOVINE SERUM ALBUMIN FOR CAPILLARY ELECTROCHROMATOGRAPHY[J]. http://dx.doi.org/10.1080/00032719.2012.689793,2012.
APA	Yao, Chuanyi,Tang, Shaokun,Yan, Chao,Yao, Hong-Mei,Tade, Moses O.,&姚传义.(2012).PREPARATION OF A MONOLITH WITH COVALENTLY BOUND BOVINE SERUM ALBUMIN FOR CAPILLARY ELECTROCHROMATOGRAPHY.http://dx.doi.org/10.1080/00032719.2012.689793.
MLA	Yao, Chuanyi,et al."PREPARATION OF A MONOLITH WITH COVALENTLY BOUND BOVINE SERUM ALBUMIN FOR CAPILLARY ELECTROCHROMATOGRAPHY".http://dx.doi.org/10.1080/00032719.2012.689793 (2012).