基于UPLC-oaTOF-MS的糖尿病及糖尿病肾病的代谢组学研究

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	基于UPLC-oaTOF-MS的糖尿病及糖尿病肾病的代谢组学研究
	张洁 ; 严丽娟 ; 林琳 ; 陈文贵 ; 宋秀宇 ; 颜晓梅 ; 杭纬 ; 黄本立
刊名	http://epub.cnki.net/grid2008/brief/detailj.aspx?filename=GDXH200811011&dbname=CJFQ2008
	2008-11-10
关键词	糖尿病糖尿病肾病代谢组学超高效液相色谱飞行时间质谱
英文摘要	Ultra performance liquid chromatography(UPLC) coupled with orthogonal acceleration time-of-flight(oaTOF) mass spectrometry is becoming an effective and high-throughput analytical technique in metabonomics research.In this paper,an UPLC-oaTOF-MS system was employed to investigate the serum profiles of 40 diabete mellitus(DM)/diabetic nephropathy(DN) patients and 25 healthy volunteers in an effort to find potential biomarkers.The UPLC system produced information-rich chromatograms with typical measured peak widths of 4 s,and generated peak capacities of 225 in 15 min.After the UPLC-oaTOF-MS analysis,principal component analysis(PCA) was used for group differentiation and marker selection.In the projection of positive mode,DM/DN patients and the controls were obviously divided into three groups.Furthermore,two potential markers were identified as phytosphingosine and sphinganine,respectively.Phytosphingosine and sphinganine with decreased concentrations in the patients,were closely related to the sphingolipid me...
语种	中文
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/19969]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	张洁,严丽娟,林琳,等. 基于UPLC-oaTOF-MS的糖尿病及糖尿病肾病的代谢组学研究[J]. http://epub.cnki.net/grid2008/brief/detailj.aspx?filename=GDXH200811011&dbname=CJFQ2008,2008.
APA	张洁.,严丽娟.,林琳.,陈文贵.,宋秀宇.,...&黄本立.(2008).基于UPLC-oaTOF-MS的糖尿病及糖尿病肾病的代谢组学研究.http://epub.cnki.net/grid2008/brief/detailj.aspx?filename=GDXH200811011&dbname=CJFQ2008.
MLA	张洁,et al."基于UPLC-oaTOF-MS的糖尿病及糖尿病肾病的代谢组学研究".http://epub.cnki.net/grid2008/brief/detailj.aspx?filename=GDXH200811011&dbname=CJFQ2008 (2008).