QQ客服 官方微博 反馈留言

	Chemically Modified PPyCOOH Microtubes as an Affinity Matrix for Protein Purification
	Shi, Wei ; Shi W(石巍) ; Cao, Huihui ; Shen, Yuqing ; Song, Cunfeng ; Li, Donghui ; Zhang, Ye ; Ge, Dongtao ; Ge DT(葛东涛)
	2009-08
关键词	adsorption conducting polymers hemoglobin metal chelates microtubes
英文摘要	Novel affinity microtubes, with high ligand content and adsorption capacity of proteins, were fabricated by functionalization of the carboxyl group on the inner and outer surfaces of conductive polymer, poly(1(2-carboxyethyl)pyrrole) (PPyCOOH) microtubes with iminodiacetic acid (IDA), which affords the active site to form metal-IDA chelates used for immobilized-metal affinity chromatography. After immobilization with copper ions, the microtubes were used to recover a model protein, bovine hemoglobin, from red cell lysate in a batch manner. The protein adsorption indicates that the affinity microtubes exhibit a high adsorption capacity and good selectivity, and the adsorption isotherm fitted the Freundlich model well. Elution of the protein showed that the desorption ratio was up to 99.5% in 3 h, using 0.2 M. CH3COOH-CH3COONa buffer solution (pH = 3.6) as the desorption agent. The adsorption-desorption cycle was repeated 10 times using the same microtubes without significant loss in the hemoglobin adsorption capacity.; National Nature Science Foundation of China [30870648, 30870617, 30500127]; Natural Science Foundation of Fujian Province [C0510005]
语种	英语
出版者	WILEY-V C H VERLAG GMBH
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1002/macp.200900186]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Shi, Wei,Shi W,Cao, Huihui,et al. Chemically Modified PPyCOOH Microtubes as an Affinity Matrix for Protein Purification[J],2009.
APA	Shi, Wei.,石巍.,Cao, Huihui.,Shen, Yuqing.,Song, Cunfeng.,...&葛东涛.(2009).Chemically Modified PPyCOOH Microtubes as an Affinity Matrix for Protein Purification..
MLA	Shi, Wei,et al."Chemically Modified PPyCOOH Microtubes as an Affinity Matrix for Protein Purification".(2009).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。