QQ客服 官方微博 反馈留言

	Optimization of HPLC-APCI-MS conditions for the qualitative and quantitative determination of total solanesol in tobacco leaves
	Chen, Junhui（Ocean Univ China, Coll Chem & Chem Engn） ; Liu, Jie ; Lee, Frank Sen-Chun（First Inst Oceanog SOA, QingDao Key Lab Analyt Technol Dev & Standardizat） ; Wang, Xiaoru ; Wang XR(王小如)
	2007-12
关键词	atmospheric pressure chemical ionization mass spectrometry high-performance liquid chromatography solanesol tobacco leaves
英文摘要	HPLC coupled online with atmospheric pressure chemical ionization MS (APCI-MS) technique was evaluated for the qualitative and quantitative determination of solanesol in extracts of tobacco leaves. The solanesol and other compounds in the extract were separated on an Alltima C-8 (4.6 mm x 250 mm) column with methanol and water (98:2 v/v) as mobile phase, with flow rate of 0.8 mL/min and UV detection wavelength of 211 nm. In the APCI(+) mode, abundant stable [M-H2O + H](+) ion (m/z at 613.5) was observed, with low abundance of other fragmentation ions. A comparison of APCI-MS and ESI-MS techniques showed that APCI mode is more sensitive than ESI mode, and thus better suited for solanesol analysis. When comparing UV 211 nm and APCI-MS in SIM for solanesol quantification, the former offered better precision and reproducibility, but the latter was more than 200 times sensitive in detection. The developed method has been successfully applied to the analysis and comparison of solanesol concentration in different tobacco leaf samples.
语种	英语
出版者	WILEY-V C H VERLAG GMBH
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1002/jssc.200700239]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Chen, Junhui,Liu, Jie,Lee, Frank Sen-Chun,et al. Optimization of HPLC-APCI-MS conditions for the qualitative and quantitative determination of total solanesol in tobacco leaves[J],2007.
APA	Chen, Junhui,Liu, Jie,Lee, Frank Sen-Chun,Wang, Xiaoru,&王小如.(2007).Optimization of HPLC-APCI-MS conditions for the qualitative and quantitative determination of total solanesol in tobacco leaves..
MLA	Chen, Junhui,et al."Optimization of HPLC-APCI-MS conditions for the qualitative and quantitative determination of total solanesol in tobacco leaves".(2007).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。