| 温度骤变对皱纹盘鲍与西氏鲍及其杂交群体的热休克蛋白表达影响; Effect of Acute Temperature Change on the Expression of HSPs in Haliotis discus hannai,Haliotis gigantea and Their Hybrids | |
| 梁爽 ; 骆轩 ; 游伟伟 ; 柯才焕 | |
| 2013-09-28 | |
| 关键词 | 杂交 皱纹盘鲍 西氏鲍 热休克蛋白 hybridization Haliotis discus hannai Haliotis gigantea HSPs |
| 英文摘要 | 杂交是改良种质的有效方法,杂交后代往往具有生长率、存活率、抗逆性方面的优势.为研究杂交对鲍的耐热性的影响,以皱纹盘鲍(HAlIOTIS dISCuS HAnnAI)、西氏鲍(HAlIOTIS gIgAnTEA)以及它们的正反交f1代为研究材料,以半定量PCr为研究手段研究了在梯度温度应激下,2种热休克蛋白(HSPS)在4个群体中的表达情况.实验结果表明,在正常温度下,西氏鲍具有最高的HSPS水平;在应激条件下,皱纹盘鲍的HSPS水平呈现先上升后下降的趋势,其他3个群体的HSPS水平均随温度上升而上升;在相对高温的30℃条件下,皱纹盘鲍的HSPS水平显著低于其他3个群体.本研究的结果说明,正反交f1代明显地继承了西氏鲍的耐高温能力,表现出杂种优势或中亲优势.; Recently,disease outbreaks related with severe temperaturein abalone Haliotis discus hannaihad happened frequently,which is probably owing to genetical characterization decline.Hybridization is an effective way of genetic improvement which could bring advanced traits to hybrids.To investigate the effect of hybridization on thermal tolerance ability in abalone,we used RT-PCR to study the expression of HSPs in H.discus hannai,Haliotis gigantea and their hybriding populations when exposed to diffierent temperatures.Results indicated that,H.giganteahad the highest HSPs levels at control temperature;Under stress conditions,HSPs levels in H.discus hannai showed as "first rose then fall",while HSPs levels in other 3groups rose as the temperature rose;At 30℃,HSPs levels in H.discus hannai was the lowest.This study proved heterosis brought by interspecies at the molecule level.; 国家高技术研究发展计划(863)项目(2012AA10A412); 国家自然科学基金项目(41106120); 国家星火计划项目(2011GA720001); 广东省教育部产学研结合项目(2012B091100085); 福建省产业技术开发项目(闽发改投资(2010)983号); 福建省科技重点项目(2011N0033); 福建省产学研重大项目(2012N5012) |
| 语种 | zh_CN |
| 内容类型 | 期刊论文 |
| 源URL | [http://dspace.xmu.edu.cn/handle/2288/104646]  |
| 专题 | 海洋环境－已发表论文 |
| 推荐引用方式 GB/T 7714 | 梁爽,骆轩,游伟伟,等. 温度骤变对皱纹盘鲍与西氏鲍及其杂交群体的热休克蛋白表达影响, Effect of Acute Temperature Change on the Expression of HSPs in Haliotis discus hannai,Haliotis gigantea and Their Hybrids[J],2013. |
| APA | 梁爽,骆轩,游伟伟,&柯才焕.(2013).温度骤变对皱纹盘鲍与西氏鲍及其杂交群体的热休克蛋白表达影响.. |
| MLA | 梁爽,et al."温度骤变对皱纹盘鲍与西氏鲍及其杂交群体的热休克蛋白表达影响".(2013). |
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