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雄性三疣梭子蟹神经器官GnRH受体的免疫组织化学研究; Immunohistochemical Study of GnRH Receptor in the Nervous Organs of the Male Swimming Crab,Portunus trituberculatus
尚丽丽 ; 冯碧云 ; 叶海辉 ; 黄辉洋 ; 李少菁
2011
关键词GnRH受体 三疣梭子蟹 神经器官 免疫组织化学 gonadotropin-releasing hormone receptor Portunus trituberculatus nervous organs immunohistochemistry
英文摘要采用MAXVISIOnTM免疫组织化学方法,以兔抗人gnrH受体的多克隆抗体,对雄性三疣梭子蟹(POrTunuS TrITubErCulATuS)的神经器官(视神经节、脑神经节和胸神经团)进行免疫组织化学定位.结果显示:gnrH受体的免疫阳性物质在雄性三疣梭子蟹视神经节、脑神经节和胸神经团的多个部位都有较为广泛的存在,定位在神经细胞的细胞质和神经髓质中,不同神经器官gnrH受体的免疫阳性强度有一定的差异.三疣梭子蟹神经器官存在gnrH受体的免疫阳性物质,为gnrH参与其神经调节作用提供了形态学依据.; The immunohistochemical technique of MaxVisionTM method and human GnRH receptor antibody against rabbit were used in this study to find out the locations of immunoreactive substances in the nervous organs of the male swimming crab,Portunus trituberculatus.The results revealed that:the immunoreactive substances of GnRH receptor were widely detected in many parts of the optic ganglion,cerebral ganglion and thoracic ganglion mass of the male crab.The immunoreactive substances located in the cytoplasm of neurosecretory cells and the neuropils.In addition,there were differentions in the intensity of immunoreaction in these three nervous organs.The immunoreactive substances of GnRH receptor existed in the nervous organs could provide morphologic evidence for GnRH participating in the neuroendocrine regulation in the swimming crab.; 国家自然科学基金项目(40776084;41076081)
语种zh_CN
内容类型期刊论文
源URL[http://dspace.xmu.edu.cn/handle/2288/104418]  
专题海洋环境-已发表论文
推荐引用方式
GB/T 7714
尚丽丽,冯碧云,叶海辉,等. 雄性三疣梭子蟹神经器官GnRH受体的免疫组织化学研究, Immunohistochemical Study of GnRH Receptor in the Nervous Organs of the Male Swimming Crab,Portunus trituberculatus[J],2011.
APA 尚丽丽,冯碧云,叶海辉,黄辉洋,&李少菁.(2011).雄性三疣梭子蟹神经器官GnRH受体的免疫组织化学研究..
MLA 尚丽丽,et al."雄性三疣梭子蟹神经器官GnRH受体的免疫组织化学研究".(2011).
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