Enrichment and characterization of an anaerobic cellulolytic microbial consortium SQD-11 from mangrove soil

CORC > 厦门大学 > 海洋环境－已发表论文

	Enrichment and characterization of an anaerobic cellulolytic microbial consortium SQD-11 from mangrove soil
	Gao, Zhao-Min ; Xu, Xun ; Ruan, Ling-Wei ; Gao ZM(高兆明) ; Xu X(徐洵) ; Ruan LW(阮灵伟)
刊名	http://dx.doi.org/10.1007/s00253-013-4857-2
	2014
关键词	Cellulose Cloning Clostridium Electrophoresis Microorganisms RNA Strain
英文摘要	Enrichment of microbial consortia provides an approach to simulate and investigate microbial communities in natural environments. In this study, a cellulolytic microbial consortium SQD-1.1 was enriched from mangrove soil of Qinglan port (Hainan, China) by 27 times continuous subcultivation under anaerobic static conditions. The consortium could completely degrade 0.2 % (w/v) filter paper within 3 days and utilized it as the sole carbon source. PCR-denaturing gradient gel electrophoresis analysis revealed a stable microbial community structure in the incubation process of 10 days and in the procedure of subcultivation. Twenty-four operational taxonomic units belonging to seven phyla were obtained from the full-length 16S rRNA gene library. Five clones, closest related to the genera Alkaliflexus, Clostridium, Alistipes, Spirochaeta, and Trichococcus, were the predominant ones. Among them, M117, phylogeneticly showing high similarity (16S rRNA gene identity, 95.3 %) with the cellulolytic anaerobic bacterium Clostridium straminisolvens CSK1T, was the potential key cellulolytic bacterium. Using the plate cultivation method, 12 strains, including one potential new species and four potential new species of new genera, were isolated. The strain P2, corresponding to the most frequently detected clone (M05) in the 16S rRNA gene library, showed both CMCase and xylanase activity and may be another important cellulolytic bacterium. The findings of cellulase activity in cell pellet and cohesion and dockerin domains in metagenome data further suggested the potential of utilization of cellulosomes by the consortium to degrade cellulose. Consortium SQD-1.1 provides a candidate for investigating the mechanism of cellulose degradation under anoxic conditions in natural environments. ? 2013 Springer-Verlag Berlin Heidelberg.
语种	英语
出版者	SPRINGER
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88289]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Gao, Zhao-Min,Xu, Xun,Ruan, Ling-Wei,et al. Enrichment and characterization of an anaerobic cellulolytic microbial consortium SQD-11 from mangrove soil[J]. http://dx.doi.org/10.1007/s00253-013-4857-2,2014.
APA	Gao, Zhao-Min,Xu, Xun,Ruan, Ling-Wei,高兆明,徐洵,&阮灵伟.(2014).Enrichment and characterization of an anaerobic cellulolytic microbial consortium SQD-11 from mangrove soil.http://dx.doi.org/10.1007/s00253-013-4857-2.
MLA	Gao, Zhao-Min,et al."Enrichment and characterization of an anaerobic cellulolytic microbial consortium SQD-11 from mangrove soil".http://dx.doi.org/10.1007/s00253-013-4857-2 (2014).