Combined effects of acute thermal and hypo-osmotic stresses on osmolality and hsp70, hsp90 and sod expression in the sea cucumber Apostichopus japonicus Selenka

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	Combined effects of acute thermal and hypo-osmotic stresses on osmolality and hsp70, hsp90 and sod expression in the sea cucumber Apostichopus japonicus Selenka
	Wang, Qing-lin ; Yu, Shan-shan ; Qin, Chuan-xin ; Dong, Shuang-lin ; Dong, Yun-wei ; Wang QL(王青林) ; Dong YW(董云伟)
刊名	http://dx.doi.org/10.1007/s10499-013-9734-6
	2014
关键词	HEAT-SHOCK ANTIOXIDANT DEFENSES MOLECULAR CHAPERONES OSMOREGULATION TEMPERATURE THERMOTOLERANCE RESPONSES GROWTH EVOLUTIONARY PHYSIOLOGY
英文摘要	National Basic Research Program of China [2013CB956500]; Nature Science funds for Distinguished Young Scholars of Fujian Province, China [2011J06017]; National Natural Science Foundation of China [41076083, 41276126]; Fundamental Research Funds for the Central Universities; Program for New Century Excellent Talents in University of Fujian Province; China Postdoctoral Science Foundation [2013M541862]; open funds of Scientific Observing and Experimental Station of South China Sea Fishery Resources and Environment, Ministry of Agriculture, P. R. China [SSCS-201207]; The combined effects of acute temperature and salinity on osmolality, expressions of heat shock proteins mRNA (hsp70, hsp90a and hsp90b) and superoxide dismutase mRNA (sod) were investigated in the sea cucumber Apostichopus japonicus Selenka. There were 12 treatments (combinations of temperature at 16, 20, 24 and 28 A degrees C and salinity at 22, 27 and 32 ppt). In low salinity environments, the osmolality of the sea cucumber's coelomic fluid decreased immediately and reached osmotic balance within 6 h. The decline of osmolality after 2 h of hypo-osmotic stress was faster at high temperatures (28 A degrees C) than that at low temperatures (16 and 20 A degrees C). Cellular level stress was indicated by up-regulation of hsp70, hsp90s and sod mRNA, and the maximal expression of all genes occurred at 6 h after stresses. The up-regulation of hsps and sod mRNA indicated the emergence of protein denaturation and oxidative damage and also suggested an increase in energy consumption at high temperature and low salinity. These results indicated that high temperature and low salinity could change biochemical pathways and energy budgets and then potentially impair the osmoregulation of the sea cucumber. Therefore, effective ways should be taken (e.g., draining off the upper freshwater, exchanging water and adding man-made sea water) to prevent the damage to sea cucumber culture caused by low salinity induced by rainstorms, especially at high temperature.
语种	英语
出版者	SPRINGER
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88127]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Wang, Qing-lin,Yu, Shan-shan,Qin, Chuan-xin,et al. Combined effects of acute thermal and hypo-osmotic stresses on osmolality and hsp70, hsp90 and sod expression in the sea cucumber Apostichopus japonicus Selenka[J]. http://dx.doi.org/10.1007/s10499-013-9734-6,2014.
APA	Wang, Qing-lin.,Yu, Shan-shan.,Qin, Chuan-xin.,Dong, Shuang-lin.,Dong, Yun-wei.,...&董云伟.(2014).Combined effects of acute thermal and hypo-osmotic stresses on osmolality and hsp70, hsp90 and sod expression in the sea cucumber Apostichopus japonicus Selenka.http://dx.doi.org/10.1007/s10499-013-9734-6.
MLA	Wang, Qing-lin,et al."Combined effects of acute thermal and hypo-osmotic stresses on osmolality and hsp70, hsp90 and sod expression in the sea cucumber Apostichopus japonicus Selenka".http://dx.doi.org/10.1007/s10499-013-9734-6 (2014).