Identification and characterization of Vibrio harveyi associated with diseased abalone Haliotis diversicolor

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	Identification and characterization of Vibrio harveyi associated with diseased abalone Haliotis diversicolor
	Jiang, Qingru ; Shi, Liuyang ; Ke, Caihuan ; You, Weiwei ; Zhao, Jing ; Ke CH(柯才焕) ; You WW(游伟伟) ; Zhao J(赵晶)
刊名	http://dx.doi.org/10.3354/dao02572
	2013
关键词	MULTILOCUS SEQUENCE-ANALYSIS CULTURED SMALL ABALONE 16S RIBOSOMAL-RNA SUPERTEXTA LISCHKE MASS MORTALITY GENUS VIBRIO PATHOGEN MISIDENTIFICATION ALGINOLYTICUS CAMPBELLII
英文摘要	National Natural Science Foundation of China [31202014, 31101896]; Hi-Tech Research and Development (863) Program of China [2012AA10A412]; Mass mortality of farmed small abalone Haliotis diversicolor occurred in Fujian, China, from 2009 to 2011. Among isolates obtained from moribund abalones, the dominant species AP37 exhibited the strongest virulence. After immersion challenge with 10(6) CFU ml(-1) of AP37, abalone mortalities of 0, 53 and 67% were induced at water temperatures of 20 degrees C, 24 degrees C, and 28 degrees C, respectively. Following intramuscular injection, AP37 showed a low LD50 (median lethal concentration) value of 2.9 x 10(2) CFU g(-1) (colony forming units per gram abalone wet body weight). The LT50 (median lethal time) values were 5.2 h for 1 x 10(6) CFU abalone(-1), 8.4 h for 1 x 10(5) CFU abalone(-1), and 21.5 h for 1 x 10(4) CFU abalone(-1). For further analysis of virulence, AP37 was screened for the production of extracellular factors. The results showed that various factors including presence of flagella and production of extracellular enzymes, such as lipase, phospholipase and haemolysin, could be responsible for pathogenesis. Based on its 16S rRNA gene sequence, strain AP37 showed >98.8% similarity to Vibrio harveyi, V. campbellii, V. parahaemolyticus, V. alginolyticus, V. natriegens and V. rotiferianus, so it could not be identified by this method. However, multi-locus sequence analysis (MLSA) of concatenated sequences, including the rpoD, rctB, gyrB, toxR and pyrH genes, identified strain AP37 as V. harveyi. Phenotypic characters of AP37 were identified by API 20E. In antibiotic susceptibility tests, strain AP37 exhibited susceptibility to 7 antibiotics and resistance to 13. This is the first report of a V. harveyi-related species being linked with the mass mortality of adult abalone H. diversicolor in southern China.
语种	英语
出版者	INTER-RESEARCH
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88062]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Jiang, Qingru,Shi, Liuyang,Ke, Caihuan,et al. Identification and characterization of Vibrio harveyi associated with diseased abalone Haliotis diversicolor[J]. http://dx.doi.org/10.3354/dao02572,2013.
APA	Jiang, Qingru.,Shi, Liuyang.,Ke, Caihuan.,You, Weiwei.,Zhao, Jing.,...&赵晶.(2013).Identification and characterization of Vibrio harveyi associated with diseased abalone Haliotis diversicolor.http://dx.doi.org/10.3354/dao02572.
MLA	Jiang, Qingru,et al."Identification and characterization of Vibrio harveyi associated with diseased abalone Haliotis diversicolor".http://dx.doi.org/10.3354/dao02572 (2013).